In vitro effects of some anthelminthics on the malate dehydrogenase and lactate dehydrogenase enzyme activities of Taenia saginata

Abstract

Malate Dehydrogenase (MDH) and Lactate Dehydrogenase (LDH) activities were demonstrated in the mitochondrial and cytosolic fractions of an intestinal cestode, Taenia saginata, and then the in vitro effects of three anthelminthics, albendazole, niclosamide, and piperazine on these enzymes were investigated. The Vmax and Km values of MDH were found to be 3.00 ?mol (min mg protein)-1 and 0.0166 mM respectively. In vitro addition of albendazole and niclosamide increased the Vmax value of MDH, whereas it decreased Km value of MDH (p<0.005). The Vmax and Km values of LDH were found to be 0.028 ?mol (min mg protein)-1 and 0.055 mM respectively. Albendazole and niclosamide solutions increased the Vmax value of LDH (p<0.05). Km value of LDH was reduced albendazole and niclosamide solutions (P<0.05). Piperazine affected neither MDH nor LDH activities. These results suggest that the activity of MDH and LDH in T. saginata are activated and the carbohydrate metabolism of this parasite is changed by albendazole and niclosamide.