Cytochromee P-450 reductase activities in the liver microsomes of diabetic rats induced with some chemical solvents

Abstract

Cytochrome P-450 Reductase enzyme is an important enzyme providing electrons to the microsomal cytochrome P-450 enzyme system metabolizing some endogenous subtances forming in the liver, drugs, chemical carcinogens insecticides and food preservation materials. In this work, it has been attempted to determine the alterations brought about in the enzyme activities by oral administering acetone, isopropanol and ethanol to chemically induced diabetic rats. The rats in whom diabetes was induced diabetic by 65 mg/kg streptozotocin (stz) were divided into four main groups as a stz alone (group I), stz + aceton (group II), stz + isopropanol (group III), stz + ethanol (group IV) respectively. The control group was given physiological saline. Then liver microsomal fractions were obtained both from the control and experimental groups of rats. The enzyme activities were determined by using Strobel's method. As a result of these investigations the cytochrome P-450 reductase activities in the controls was determined to be 2,41 U/mg protein. It was observed that compared to that of the control group the enzyme were activated by 95% in the first group, 286% in Group II, 205% in Group III and 97% in Group IV.