The investigation of Entamoeba histolytica prevalence in some villages of Sivas by ELISA method.
Abstract
Humans may be infected with three morphologically identical Entamoeba species; pathogenic E. histolytica, commensal E. moshkowskii and E. dispar. The aim of the present study was to determine the true prevalence of the E. histolytica using native lugol, trichrome staining and a monoclonal antigen detection kit (ELISA kit E. histolytica-II; Techlab, Inc., Blacksburg, VA) among primary school children living in the rural areas around Sivas. A total of 1449 stool samples were examined by native lugol and Trichrome staining, and 312 (22%) samples were positive for one or more parasite species. Additionally, 22 (1.5%) stool samples were found to be positive for the presence of E. histolytica/dispar cysts, and these samples were further examined by E. histolytica specific antigen based ELISA. As a result, ELISA test gave negative reactions for all the samples. Also, there was no cross reaction with other luminal protozoa such as E. coli, G. intestinalis, B. hominis and I. butschlii. The data reveals that E. histolytica prevalence may be lower than estimated.
Source
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derne?i = Acta parasitologica Turcica / Turkish Society for ParasitologyVolume
35Issue
1Collections
- Makale Koleksiyonu [5745]