dc.contributor.author | Özan F. | |
dc.contributor.author | Polat Z.A. | |
dc.contributor.author | Tepe B. | |
dc.contributor.author | Er K. | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:31:23Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:31:23Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 1526-3711 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/5212 | |
dc.description | Jaypee Brothers Medical Publishers (P) Ltd | en_US |
dc.description.abstract | Aim: The purpose of this study was to determine the ability of Salvia officinalis (S. officinalis) extracts to serve as a storage medium for the maintenance of periodontal ligament (PDL) cell viability of avulsed teeth. Methods and Materials: PDL cells were obtained from healthy third molars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). Cultures were subjected to 4, 2.5, 1.5, and 0.5% S. officinalis solutions, Hank's balanced salt solution (HBSS), phosphate buffered saline (PBS), and tap water. Tissue culture plates were incubated with experimental media at 37°C for 1, 3, 6, 12 or 24 hours. PDL cell viability was assessed by trypan blue exclusion. Statistical analysis of the data was performed by one-way analysis of variance (ANOVA) complemented by the Tukey's test. The level of significance was 5% (p< 0.05). Results: The results showed 2.5% S. officinalis was a more effective storage medium than the other experimental solutions (p<0.05). Only at 1 hour and 3 hours was there found similar effect between 2.5% S. officinalis and HBSS. At 24 hours, 2.5% S. officinalis was found to be significantly better than the other solutions tested. Conclusion: S. officinalis can be recommended as a suitable transport medium for avulsed teeth. Clinical Significance: The findings of this study support the use of S. officinalis as another option for clinicians to use to store and transport avulsed teeth until reimplantation procedures can be done. | en_US |
dc.description.sponsorship | Özan, F.; Department of Oral and Maxillofacial Surgery, School of Dentistry, Cumhuriyet University, Sivas, Turkey; email: fozan@cumhuriyet.edu.tr | en_US |
dc.language.iso | eng | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Avulsed teeth | en_US |
dc.subject | Cell culture | en_US |
dc.subject | PDL cells | en_US |
dc.subject | Salvia officinalis | en_US |
dc.subject | Storage media | en_US |
dc.title | Influence of storage media containing Salvia officinalis on survival of periodontal ligament cells | en_US |
dc.type | article | en_US |
dc.relation.journal | Journal of Contemporary Dental Practice | en_US |
dc.contributor.department | Özan, F., Department of Oral and Maxillofacial Surgery, School of Dentistry, Cumhuriyet University, Sivas, Turkey, Turkish Association of Oral and Maxillofacial Surgeons -- Polat, Z.A., CUTFAM Research Center, School of Medicine, Cumhuriyet University, Sivas, Turkey -- Tepe, B., Department of Molecular Biology and Genetics, School of Science and Literature, Cumhuriyet University, Sivas, Turkey -- Er, K., Department of Endodontics, Faculty of Dentistry, Karadeniz Technical University, Trabzon, Turkey | en_US |
dc.identifier.volume | 9 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.endpage | 24 | en_US |
dc.identifier.startpage | 17 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |