Show simple item record

dc.contributor.authorPolat Z.A.
dc.contributor.authorWalochnik J.
dc.contributor.authorObwaller A.
dc.contributor.authorVural A.
dc.contributor.authorDursun A.
dc.contributor.authorArici M.K.
dc.date.accessioned2019-07-27T12:10:23Z
dc.date.accessioned2019-07-28T09:32:08Z
dc.date.available2019-07-27T12:10:23Z
dc.date.available2019-07-28T09:32:08Z
dc.date.issued2014
dc.identifier.issn1442-6404
dc.identifier.urihttps://dx.doi.org/10.1111/ceo.12120
dc.identifier.urihttps://hdl.handle.net/20.500.12418/5464
dc.descriptionBlackwell Publishingen_US
dc.description.abstractBackground: In this study, a series of compounds - miltefosine, polyhexamethylene biguanide, chlorhexidine and propamidine isethionate - and combinations of the latter three agents with miltefosine were prepared and used in a rat model for the topical treatment of Acanthamoeba keratitis. Methods: The corneas of rats were infected with Acanthamoeba hatchetti. On the fifth day, all corneas were microscopically examined in order to determine the grade of infections. Nine groups were then prepared: miltefosine (65.12?g/mL); chlorhexidine (0.02%); polyhexamethylene biguanide (0.02%), propamidine isethionate (0.1%), miltefosine plus chlorhexidine, miltefosine plus polyhexamethylene biguanide; miltefosine plus propamidine isethionate; infected control; and a non-infected control group. The treatment was continued for 28 days. After the treatment, the corneas were excised and used for Acanthamoeba culture to investigate the presence of Acanthamoeba growth. For the determination of cytotoxicity of the drugs on L929 cells, colorimetric assays were performed. Results: The best treatment results were obtained from the polyhexamethylene biguanide plus miltefosine group; the ratio of fully recovered eyes was 28.4%. It was proven that the miltefosine-polyhexamethylene biguanide combination yielded the highest anti-acanthamoebal activity in that approximately 86% of the eyes were cleared from amoebae. The cytotoxicity values of the miltefosine and the control groups were compared with other groups and found to be statistically different (P<0.05). Conclusion: This in vivo study demonstrates that a miltefosine-polyhexamethylene biguanide combination is highly effective for the treatment of Acanthamoeba keratitis. © 2013 Royal Australian and New Zealand College of Ophthalmologists.en_US
dc.description.sponsorshipPolat, Z.A.; Department of Medical Parasitology, Cumhuriyet University School of Medicine, 58140 Sivas, Turkey; email: zubeydeakin@yahoo.comen_US
dc.language.isoengen_US
dc.relation.isversionof10.1111/ceo.12120en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAcanthamoebaen_US
dc.subjectKeratitisen_US
dc.subjectMiltefosineen_US
dc.subjectPolyhexamethylene biguanideen_US
dc.subjectRat keratitis modelen_US
dc.titleMiltefosine and polyhexamethylene biguanide: A new drug combination for the treatment of Acanthamoeba keratitisen_US
dc.typearticleen_US
dc.relation.journalClinical and Experimental Ophthalmologyen_US
dc.contributor.departmentPolat, Z.A., Department of Medical Parasitology, Cumhuriyet University School of Medicine, Sivas, Turkey -- Walochnik, J., Institute of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Vienna, Austria -- Obwaller, A., Orphanidis Pharma Research GmbH, Vienna, Austria -- Vural, A., Department of Ophthalmology, Cumhuriyet University School of Medicine, Sivas, Turkey -- Dursun, A., Department of Ophthalmology, Cumhuriyet University School of Medicine, Sivas, Turkey -- Arici, M.K., Department of Ophthalmology, Cumhuriyet University School of Medicine, Sivas, Turkeyen_US
dc.identifier.volume42en_US
dc.identifier.issue2en_US
dc.identifier.endpage158en_US
dc.identifier.startpage151en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record