Comparing the antibacterial activity of gaseous ozone and chlorhexidine solution on a tooth cavity model
Objective: To evaluate the antibacterial activity of gaseous ozone andchlorhexidine solution on a tooth cavity model. Study Design: Twenty-one human molars were divided into 3 groups. Cavities were then cut into the teeth (4 per tooth, 28 cavities per group). After sterilization, the teeth wereleft in broth cultures of 106 colony-forming units (CFU) ml-1 of Streptococcus mutans (S. mutans) at 36°C for 48 h. The appropriate treatment followed (group A, control; group B, 2% chlorhexidine solution; and group C, 80s of treatment with ozone, and the cavities were thenfilled with compositeresin. After 72h, the restorations were removed, dentin chips were collected with an excavator, and the total number of microorganisms was determined. Results: Both of the treatments significantly reduced the number of S. mutans present compared with the control group and there was a significant difference between the all groups in terms of the amount of the microorganisms grown (p<0.05). Group B was beter than group C; andgroup C was better than group A. Moreover, it was found that the amount of the growth in thegroup of chlorhexidine was significantly less than that ofthe ozone group (p<0.05). Conclusion: Chlorhexidine solution was the antibacterial treatment most efficacious on S. mutans; however, ozone application could be an anlternative cavity disinfection method because of ozone's cavity disinfectionactivity. © Medicina Oral S.L.