Histomorphometric assessment of the impact of bovine demineralized bone graft on bone healing versus autogenous, allogeneic and synthetic grafts in experimentally- induced critical size bone defects in rats [Ratlarda deneysel olarak oluşturulan kritik boyutlu kemik defektlerine uygulanan siğir kaynakli deminarelize kemik greftininin kemik iyiyleşmesine olan etkisinin otojen, allojenik ve sentetik greftlerle karşilaştirilmasinin histomorfometrik olarak i?ncelenmesi]
Abstract
Objectives: Bone tissue has the ability to heal itself (regeneration) and may restore its morphology and function when injured. However, healing may be limited in the case of large wounds. A "critical-size defect" is an intraosseous wound in a particular bone and species of animal that will not heal spontaneously morphologically and functionally during the lifetime of the animal. Autogenous bone grafts have been regarded as "gold standard" for treatment of critical-size bone defects. Known drawbacks of autogenous bone graft have led to research efforts focusing on different graft materials and resulted in several alternative substitutes including xenografts, allografts and synthetic graft materials. The aim of the present study was to perform a histomorphometric study to investigate the effect of bovine demineralized bone graft on bone healing in comparison to autogenous, allogeneic and synthetic graft materials when applied into critical size bone defects with a diameter of 5 mm. Materials and Methods: Experimental animals were divided into 4 groups, each having 8 rats. In the control group, a mandibular defect was created and then filled with a bovine graft (Integros Bone Plus XS Adana/Turkey). In the experimental groups, autogenous bone was reinserted into the critical-size defect which was created using a trephine bur in Group I (autogenous group) and Group II received a human graft (Korea Bone Bank (KBB) Gasandong Keumcheongu Seoul/South Korea) to fill the critical-size defect. For Group III, a synthetic bone graft ?-tricalcium phosphate (Cerasorb North Carolina/USA) was applied on the critical-size bone defect. Specimens were obtained for histomorphometric examination and rats were sacrificed on day 28. Results: Histomorphometric examination performed on day 28 to evaluate the relative effects of different graft materials on new bone formation showed no significant difference in the volume of newly formed bone between groups receiving autogenous bone graft, allograft and bovine xenograft but a significant difference was observed versus synthetic bone graft group. Conclusion: While autogenous bone graft is currently regarded as the gold standard for bone regeneration, the difficulties in harvesting and application of autografts limit their use. Our results demonstrate that bovine bone graft may be used as a safe and effective alternative to autogenous bone graft. © 2018 Cumhuriyet University Faculty of Dentistry.Amaç: Kemik dokusu iyileşme özelliğine (rejenerasyon) sahiptir ve yaralanan kemik dokusu şekil ve fonksiyonunu yeniden kazanabilmektedir. Fakat yaralanmanin boyutu büyük olduğu zaman iyileşme sinirli kalabilmektedir. Kritik boyutlu kemik defekti; kemik dokusunda, canlinin yaşami boyunca, şekil ve fonksiyon olarak, kendiliğinden tamamen iyileşmesinin mümkün olmayacaği boyuttaki defekt anlamina gelir. Kritik kemik defektlerinde tedavi için otojen greft uygulamasi altin standart olarak kabul edilir. Otojen kemik greftinin bazi dezavantajlari nedeniyle araştirmacilar çalişmalarini farkli greft materyalleri üzerinde yoğunlaştirmişlardir. Bu çalişmalar neticesinde Ksenogreft, Allogreft ve sentetik greft materyalleri gibi seçenekler ortaya çikmiştir. çalişmamizin amaci 5mm çapinda kritik boyutlu kemik defektlerinde siğir kaynakli demineralize kemik grefti uygulamasinin kemik iyileşmesine etkisi ile ayni çaptaki defektlere otojenik, allojenik ve sentetik greft materyali uygulandiği zaman elde edilen iyileşmelerin histomorfometrik olarak incelenmesidir. Gereç ve Yöntemler: Deney hayvanlari her grup 8 deney hayvanindan oluşan 4 gruba ayrildi. Kontrol grubunda mandibulada defekt oluşturulduktan sonra defekt siğir kaynakli kemik grefti (Integros Bone Plus XS Adana/Türkiye) ile dolduruldu. Daha sonraki deney gruplarinda; I. grupta oluşturulan kritik boyutlu defekte trefin frezle çikartilan otojen kemik tekrar konuldu. II. grupta oluşturulan kritik boyutlu defekte insan kaynakli kemik grefti (Korea Bone Bank (KBB) Gasandong Keumcheongu Seoul/Korea) uygulandi. III. grupta oluşturulan kritik boyutlu kemik defektine ise sentetik kemik grefti grubunda yer alan ?-trikalsiyum fosfat (Cerasorb North Caroline/USA) uygulandi. 28 gün sonra ratlar öldürüldü.Her grup sakrifiye edilerek histomorfometrik incelemeye alindi. Bulgular: Farkli greft materyallerinin 28. günde yeni kemik oluşumuna olan etkisinin histomorfometrik olarak incelendiğinde otojen kemik grefti, allogreft ve siğir kaynakli kemik grefti uygulanan gruplar arasinda yeni oluşan kemik hacmi bakimindan anlamli bir fark bulunmazken, sentetik kemik grefti uygulanan grupla aralarindaki fark anlamli bulunmuştur. Sonuç: Otojen kemik grefti günümüzde hala altin standart olarak kabul edilmesine rağmen, elde edilmesi ve uygulanmasindaki zorluklar nedeniyle çalişmamizda kullandiğimiz siğir kaynakli kemik greftinin otojen kemik greftine alternatif olarak güvenilir ve etkili biçimde kullanilabileceği belirlenmiştir. © 2018 Cumhuriyet University Faculty of Dentistry.
Source
Cumhuriyet Dental JournalVolume
21Issue
4Collections
- Makale Koleksiyonu [5745]
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