dc.contributor.author | Cevik, Ozge | |
dc.contributor.author | Turut, Fatma Aysun | |
dc.contributor.author | Acidereli, Hilal | |
dc.contributor.author | Yildirim, Sahin | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:37:13Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:37:13Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0250-4685 | |
dc.identifier.issn | 1303-829X | |
dc.identifier.uri | https://dx.doi.org/10.1515/tjb-2017-0355 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/5981 | |
dc.description | WOS: 000461427300007 | en_US |
dc.description.abstract | Background: Potential targets for prostate cancer therapy are urgently needed for curative of patients. Cyclosporine-A (CsA), an immunosuppressive and a selective cyclooxygenase-2 (COX-2) inhibitor, exerts antitumor activity. However, the molecular effects of CsA is not fully understood in prostate cancer. In this research, we sought to determine role and mechanism of CsA in prostate cancer. Materials and methods: PC3 and DU145 cells were treated with CsA time (12, 24, 48 h) and dose dependent (2.5, 10, 25 mu M) and cell survival, migration, colony formation, expression of apoptosis related proteins/genes using MTT assay, scratch assay, Western blotting/qPCR. At the same time, cells treated with CsA to test on the effects of COX-2 promoter activity using luciferase reporter plasmid. Lastly, functional role in the CsA treatment prostate cancer cells were interrogated for relationship of TGF beta, Akt, caspases and COX-2. Results: These study findings provided direct evidences that the CsA induced apoptosis and downregulated migration. Conclusions: CsA downregulated Akt as well as COX-2 and upregulated TGF beta, resulting in the suppression of cell migration which was augmented a potential therapeutic of CsA in prostate cancer cells. | en_US |
dc.description.sponsorship | Cumhuriyet University Research Grant [ECZ-003/ECZ-008]; Scientific and Technological Research Council of Turkey (TUBITAK) [214Z057]; Science Academy's Young Scientist Award (BAGEP)-2016 | en_US |
dc.description.sponsorship | The authors are grateful to Dr. Neerja Kaushik Basu for the kind gift of p-COX-2-Luc reporter plasmids. Authors would like to thank Mustafa Ergul and H. Eren Bostanci from Faculty of Pharmacy in Cumhuriyet University. This work was supported by the Cumhuriyet University Research Grant (Projects ECZ-003/ECZ-008) and by a grant (214Z057) from the Scientific and Technological Research Council of Turkey (TUBITAK) and Science Academy's Young Scientist Award (BAGEP)-2016 to Dr. Ozge Cevik. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | WALTER DE GRUYTER GMBH | en_US |
dc.relation.isversionof | 10.1515/tjb-2017-0355 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | 5 Cyclosporine | en_US |
dc.subject | Prostate cancer | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | Migration | en_US |
dc.subject | Anti-cancer | en_US |
dc.subject | Androgen | en_US |
dc.title | Cyclosporine-A induces apoptosis in human prostate cancer cells PC3 and DU145 via downregulation of COX-2 and upregulation of TGF beta | en_US |
dc.type | article | en_US |
dc.relation.journal | TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI | en_US |
dc.contributor.department | [Cevik, Ozge] Adnan Menderes Univ, Dept Biochem, Sch Med, TR-09100 Aydin, Turkey -- [Cevik, Ozge -- Turut, Fatma Aysun -- Acidereli, Hilal] Cumhuriyet Univ, Fac Pharm, Dept Biochem, Sivas, Turkey -- [Yildirim, Sahin] Cumhuriyet Univ, Sch Med, Dept Pharmacol, Sivas, Turkey | en_US |
dc.contributor.authorID | Cevik, Ozge -- 0000-0002-9325-3757 | en_US |
dc.identifier.volume | 44 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.endpage | 54 | en_US |
dc.identifier.startpage | 47 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |