dc.contributor.author | Turan, Ibrahim | |
dc.contributor.author | Demir, Selim | |
dc.contributor.author | Kilinc, Kagan | |
dc.contributor.author | Yaman, Serap Ozer | |
dc.contributor.author | Misir, Sema | |
dc.contributor.author | Kara, Hanife | |
dc.contributor.author | Genc, Berna | |
dc.contributor.author | Mentese, Ahmet | |
dc.contributor.author | Aliyazicioglu, Yuksel | |
dc.contributor.author | Deger, Orhan | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:37:31Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:37:31Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 2095-1779 | |
dc.identifier.issn | 2214-0883 | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.jpha.2017.12.005 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/6101 | |
dc.description | WOS: 000451243800007 | en_US |
dc.description | PubMed ID: 30595946 | en_US |
dc.description.abstract | Rosa canina is a member of the genus Rosa that has long been used for medical objectives. Several studies have reported cytotoxic effects of different Rosa species, but there has been only limited investigation of the cytotoxic effect of R. canina. The purpose of the current study was to examine the potential effect of R. canina extract on cell viability, the cell cycle, apoptosis, and the expression of telomerase in human colon cancer (WiDr) cells. The cytotoxic effect of the extract was determined using MIT assay. The mechanism involved in the cytotoxic effect of the extract was then evaluated in terms of apoptosis and the cell cycle using flow cytometry. Mitochondrial membrane potential (MMP) was investigated using the fluorometric method, and expression levels of telomerase were studied using RT-PCR. R. canina extract exhibited a selective cytotoxic effect on WiDr cells compared with normal colon cells. The extract induced cell cycle arrest at the S phase and apoptosis via reduced MMP in WiDr cells. R. canina extract significantly repressed telomerase expressions at treatment times of 48 and 72 h in WiDr cells. Our results suggest that R. canina may have considerable potential for development as a novel natural product-based anticancer agent. (C) 2018 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. | en_US |
dc.description.sponsorship | Foundation of Scientific Research of Gumushane University [13.F5119.02.1] | en_US |
dc.description.sponsorship | The authors would like to thank the Foundation of Scientific Research of Gumushane University for financially supporting this research under Project No: 13.F5119.02.1. The authors also wish to thank Professor Ersan Kalay from the Medical Biology Department, Karadeniz Technical University for professional assistance with the flow cytometry studies. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.relation.isversionof | 10.1016/j.jpha.2017.12.005 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | Colon cancer | en_US |
dc.subject | Cytotoxicity | en_US |
dc.subject | Rosa canina | en_US |
dc.subject | Telomerase | en_US |
dc.title | Cytotoxic effect of Rosa canina extract on human colon cancer cells through repression of telomerase expression | en_US |
dc.type | article | en_US |
dc.relation.journal | JOURNAL OF PHARMACEUTICAL ANALYSIS | en_US |
dc.contributor.department | [Turan, Ibrahim -- Kilinc, Kagan -- Genc, Berna] Gumushane Univ, Fac Engn & Nat Sci, Dept Genet & Bioengn, TR-29100 Gumushane, Turkey -- [Turan, Ibrahim] Gumushane Univ, Med Plants Tradit Med Practice & Res Ctr, TR-29100 Gumushane, Turkey -- [Demir, Selim] Karadeniz Tech Univ, Fac Hlth Sci, Dept Nutr & Dietet, TR-61080 Trabzon, Turkey -- [Yaman, Serap Ozer -- Misir, Sema -- Kara, Hanife -- Aliyazicioglu, Yuksel -- Deger, Orhan] Karadeniz Tech Univ, Fac Med, Dept Med Biochem, TR-61080 Trabzon, Turkey -- [Misir, Sema] Cumhuriyet Univ, Fac Pharm, Dept Biochem, TR-58140 Sivas, Turkey -- [Mentese, Ahmet] Karadeniz Tech Univ, Vocat Sch Hlth Sci, Program Med Lab Tech, TR-61080 Trabzon, Turkey | en_US |
dc.contributor.authorID | Demir, Selim -- 0000-0002-1863-6280 | en_US |
dc.identifier.volume | 8 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.endpage | 399 | en_US |
dc.identifier.startpage | 394 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |