An in vitro Study of Cytotoxic Activity of Euphorbia macroclada boiss on Mcf-7 Cells
dc.contributor.author | Tas, Ayca | |
dc.contributor.author | Sahin-Bolukbasi, Serap | |
dc.contributor.author | Cevik, Esranur | |
dc.contributor.author | Ozmen, Esma | |
dc.contributor.author | Gumus, Erkan | |
dc.contributor.author | Silig, Yavuz | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:37:35Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:37:35Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0019-5464 | |
dc.identifier.uri | https://dx.doi.org/10.5530/ijper.52.4s.86 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/6123 | |
dc.description | WOS: 000454950700019 | en_US |
dc.description.abstract | Objective: The study was aimed to evaluate the cytotoxic activity of acetone extract of leaves, flower and body of Euphorbia macroclada boiss on human breast cancer cell line (MCF-7). Material: The cells were plated at a cell density of 1x10(5) cells in 96-well plates and grown with DMEM medium containing supplemented with 10% FBS and 1% penicillin. The cells were treated by different concentrations of acetone extract of Euphorbia macroclada boiss (10-1000 mu g/mL) during 24, 48 and 72 h. The cytotoxic activities of the tested compounds were determined by cell proliferation analysis using standard (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: After the evaluation of cytotoxicity assay results, it is determined that flower and body parts have a significant cytotoxic effect on MCF-7 breast cancer cell line. The values that obtained reading at 570 nm spectrophotometrically, were analyzed with GraphPad Prism7 and IC50 growth inhibition values was determined. Conclusion: The results of MTT assay showed that leaves, flower and body significantly reduced % cell viability comparative to the control. It was also shown that body had more growth inhibitory effect on MCF-7 cell compared to the leaves part. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | ASSOC PHARMACEUTICAL TEACHERS INDIA | en_US |
dc.relation.isversionof | 10.5530/ijper.52.4s.86 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Euphorbia macroclada boiss | en_US |
dc.subject | Cytotoxic activity | en_US |
dc.subject | MCF-7 | en_US |
dc.subject | Breast cancer | en_US |
dc.title | An in vitro Study of Cytotoxic Activity of Euphorbia macroclada boiss on Mcf-7 Cells | en_US |
dc.type | article | en_US |
dc.relation.journal | INDIAN JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH | en_US |
dc.contributor.department | [Tas, Ayca] Cumhuriyet Univ, Fac Hlth Sci, Dept Nutr & Diet, Sivas, Turkey -- [Sahin-Bolukbasi, Serap -- Cevik, Esranur] Cumhuriyet Univ, Fac Pharm, Dept Biochem, Sivas, Turkey -- [Ozmen, Esma -- Silig, Yavuz] Cumhuriyet Univ, Fac Med, Dept Biochem, Sivas, Turkey -- [Gumus, Erkan] Cumhuriyet Univ, Fac Med, Dept Histol, Sivas, Turkey -- [Gumus, Erkan] Cumhuriyet Univ, Fac Med, Dept Embryol, Sivas, Turkey | en_US |
dc.identifier.volume | 52 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.endpage | S123 | en_US |
dc.identifier.startpage | S119 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
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