dc.contributor.author | Nawaz, Muhammad Azhar Hayat | |
dc.contributor.author | Majdinasab, Marjan | |
dc.contributor.author | Latif, Usman | |
dc.contributor.author | Nasir, Muhammad | |
dc.contributor.author | Gokcec, Gultekin | |
dc.contributor.author | Anwar, Muhammad Waqas | |
dc.contributor.author | Hayat, Akhtar | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:37:42Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:37:42Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0731-7085 | |
dc.identifier.issn | 1873-264X | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.jpba.2018.07.005 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/6155 | |
dc.description | WOS: 000442699900047 | en_US |
dc.description | PubMed ID: 30036703 | en_US |
dc.description.abstract | In this study, a sensitive and selective electrochemical sensor was fabricated by usinga screen printed carbon electrode (SPCE), multi-walled carbon nanotubes (MWCNTs) and beta-cyclodextrin (beta-CD) for detecting cholesterol. MWCNTs were functionalized with benzoic acid moiety by employing diazonium salt chemistry, and, subsequently, a thin film of functionalized CNTs were coated on the surface of SPCE. Afterwards, beta-CD was immobilized on functionalized MWCNTs modified SPCE which acts as a host to recognize guest (cholesterol) molecule specifically. Under the optimal experimental conditions and using differential pulse voltammetry (DPV) as transduction technique the sensor was able to detect cholesterol level ranges from 1 nM to 3 mu M, with a detection limit of 0.5 nM. Specificity of the developed sensor towards target analyte (cholesterol) was confirmed in the presence of common interfering species including glucose, uric acid and ascorbic acid. The applicability of proposed sensor was also demonstrated for cholesterol determination in human serum samples with good recovery results (94-96%) and maximum RSD (relative standard deviation) of 4.5%. (C) 2018 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | HEC (Higher Education Commission of Pakistan)-NRPU project [20-4286/RD/HEC/2014] | en_US |
dc.description.sponsorship | This work was carried out under the HEC (Higher Education Commission of Pakistan)-NRPU project; No. 20-4286/R&D/HEC/2014 of Akhtar Hayat. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.relation.isversionof | 10.1016/j.jpba.2018.07.005 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Cholesterol detection | en_US |
dc.subject | Screen printed carbon electrode (SPCE) | en_US |
dc.subject | Multi-walled carbon nanotubes (MWCNTs) | en_US |
dc.subject | beta-Cyclodextrin (beta-CD) | en_US |
dc.subject | Serum sample | en_US |
dc.title | Development of a disposable electrochemical sensor for detection of cholesterol using differential pulse voltammetry | en_US |
dc.type | article | en_US |
dc.relation.journal | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | en_US |
dc.contributor.department | [Nawaz, Muhammad Azhar Hayat -- Latif, Usman -- Nasir, Muhammad -- Hayat, Akhtar] COMSATS Inst Informat Technol, Interdisciplinary Res Ctr Biomed Mat, Lahore, Pakistan -- [Majdinasab, Marjan] Shiraz Univ, Dept Food Sci & Technol, Shiraz, Iran -- [Gokcec, Gultekin] Cumhuriyet Univ, Educ Fac, Dept Elementary Sci, TR-58140 Sivas, Turkey -- [Anwar, Muhammad Waqas] COMSATS Inst Informat Technol, Dept Comp Sci, Lahore, Pakistan | en_US |
dc.contributor.authorID | Majdinasab, Marjan -- 0000-0003-4069-9832 | en_US |
dc.identifier.volume | 159 | en_US |
dc.identifier.endpage | 405 | en_US |
dc.identifier.startpage | 398 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |