Effect of Strontium Ranelate on Multiple Organ Damage in a Rat Sepsis Model

Abstract

Background: Sepsis and multiple organ dysfunction syndrome (MODS) are life-threatening conditions common in intensive care units. In this regard, studies have shown that Strontium ranelate has anti-inflammatory activity by blocking tumor necrosis factor-alpha (TNF alpha). Objectives: This study aimed to investigate the effect of Strontium ranelate on MODS in an experimental sepsis model. Methods: The study protocol was approved by Cumhuriyet University Institutional Ethics Committee for Animal Experiments (Sivas-Turkiye, date 07/12/2017). Twenty female Wistar-Albino rats were randomly divided into four groups of sham operation, cecal ligation and perforation (CLP), CLP + Strontium (S) (oral 40 mg/kg for 7 days), and S (40 mg/kg oral preoperative 5 days) + CLP + S (oral 40 mg/kg for 7 days). Blood samples were taken, and alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine were studied. Tissues were removed, and inflammation scores were determined. Results: The mean inflammation scores of lung, liver, and renal tissues were found to be the lowest in the sham group (0.8 +/- 0.45), and they increased in the S + CLP + S (1.6 +/- 0.55) and CLP + S (3.4 +/- 0.55) groups, respectively, with the highest score in the CLP (3.8 +/- 0.45) group. It was found that there was no statistical difference between the sham and S + CLP + S groups (P > 0.05); however, there was a significant difference between the other groups (P < 0.05). The mean ALT, AST, BUN and creatinine values were found to be the lowest in the sham group, and they increased in the S + CLP + S and CLP + S groups, respectively, with the highest score in the CLP group. Regarding the mean ALT results, it was noted that there was no significant difference between the sham and S + CLP + S groups (P > 0.05); however, there was a significant difference between the other groups in terms of mean ALT, and there was a significant difference between all the groups in terms of mean AST, BUN, and creatinine (P < 0.05). Conclusions: It was concluded that Strontium ranelate reduced the development of life-threatening MODS in patients with sepsis, especially when it was administered before the development of sepsis, by suppressing inflammatory mediators.