dc.contributor.author | Yaman, Turan | |
dc.contributor.author | Uyar, Ahmet | |
dc.contributor.author | Kaya, Mehmet Salih | |
dc.contributor.author | Keles, Omer Faruk | |
dc.contributor.author | Uslu, Baris Atalay | |
dc.contributor.author | Yener, Zabit | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:38:56Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:38:56Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 1984-8250 | |
dc.identifier.issn | 2175-9790 | |
dc.identifier.uri | https://dx.doi.org/10.1590/s2175-97902018000117529 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/6442 | |
dc.description | WOS: 000435137600001 | en_US |
dc.description.abstract | The present study aimed to investigate the protective effects of silymarin (SMN), an antioxidant, on methotrexate (MTX)-induced damage in rat testes. Thirty-two Wistar albino rats were divided into four groups (n = 8): control, MTX (20 mg/kg, i.p. on days 1 and 5), SMN (200 mg/kg, orally), and MTX + SMN (20 mg/kg, i.p. on days 1 and 5 and SMN 200 mg/kg orally) groups. At the end of the 6-week trial period, histopathological, immunohistochemical, biochemical, and spermatological analyses were performed on testes tissues. Histopathologically, MTX-induced damage, including depletion of germ cell and loos of spermatozoa, was significantly improved with SMN treatment. Immunohistochemically, the immunoreactivity of glutathione peroxidase 1 (GPx1) and manganese superoxide dismutase 2 (SOD2) were detected more intensely in the MTX + SMN group than in the MTX group. Biochemical examinations revealed that SMN supplementation decreased the lipid peroxidation and increased enzymatic antioxidants in the SMN-treated rats. Spermatologically, significant differences were found in the density, motility, dead-to-live sperm ratio, and abnormal sperm rate in the MTX + SMN group compared to the MTX group. In conclusion, SMN seems to have protective effects as an antioxidant against MTX-induced damage in rat testes. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | UNIV SAO PAULO, CONJUNTO QUIMICAS | en_US |
dc.relation.isversionof | 10.1590/s2175-97902018000117529 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Silymarin/effects | en_US |
dc.subject | Methotrexate/Testes/induced damages | en_US |
dc.subject | GPx1 | en_US |
dc.subject | SOD2 | en_US |
dc.subject | Pathology | en_US |
dc.title | Protective effects of silymarin on methotrexate-induced damages in rat testes | en_US |
dc.type | article | en_US |
dc.relation.journal | BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES | en_US |
dc.contributor.department | [Yaman, Turan -- Keles, Omer Faruk -- Yener, Zabit] Yuzuncu Yil Univ, Fac Vet Med, Dept Pathol, Zevve Kampusu, Van 65090, Turkey -- [Uyar, Ahmet] Mustafa Kemal Univ, Fac Vet Med, Dept Pathol, Antakya, Turkey -- [Kaya, Mehmet Salih] Ankara Yildirim Beyazit Univ, Fac Med, Dept Physiol, Ankara, Turkey -- [Uslu, Baris Atalay] Cumhuriyet Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, Sivas, Turkey | en_US |
dc.identifier.volume | 54 | en_US |
dc.identifier.issue | 1 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |