dc.contributor.author | Atas, Ahmet Duran | |
dc.contributor.author | Altay, Kursat | |
dc.contributor.author | Alim, Ahmet | |
dc.contributor.author | Ozkan, Erkan | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:39:23Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:39:23Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 1300-0128 | |
dc.identifier.uri | https://dx.doi.org/10.3906/vet-1707-93 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/6519 | |
dc.description | WOS: 000435033100005 | en_US |
dc.description.abstract | In this research, we investigate the prevalence of Dirofilaria immitis infection in dogs using microscopical, serological, and molecular methods from the Central Anatolia Region (Sivas) of Turkey and reveal the associated risk factors. Whole blood and serum samples were obtained from 306 dogs older than 7 months. Polymerase chain reaction (PCR) and the modified Knott's method (Knott) were used for detecting the microfilaria and enzyme-linked immunosorbent assay (ELISA) was applied for the presence of D. immitis antigens. The prevalence of D. immitis in dogs was detected as 2.9% (9/306) by PCR and ELISA and as 1.3% (4/306) by Knott. All Knottpositive samples were also found positive by both PCR and ELISA. According to ELISA and PCR results, no statistically significant difference was found between the prevalence in males (4.2%) and females (1.5%); dogs 0.7-2 years old (2.5%), 3-5 years old (2.2%); and more than 6 years old (7.1%); or stray (3.7%) and owned (1.7%) dogs (P > 0.05). One of the PCR products was selected for sequence analyses of the 16S small subunit rRNA gene and the obtained sequence was submitted to GenBank (KJ183078). The 16S rRNA sequence exhibited 100% identity to other D. immitis sequences available in GenBank (FJ799911, FJ799917, FJ799916). This research provides the first data on D. immitis infections in dogs from Sivas Province by using the combined identification techniques. | en_US |
dc.description.sponsorship | Scientific Research Project Fund of Cumhuriyet University [V-011] | en_US |
dc.description.sponsorship | The authors would like to thank all veterinarians and technicians for their kind help during sample collection, Dr Alparslan Yildirim for control samples, and Dr Yusuf Ziya Ograk for statistical estimation. This work was supported by the Scientific Research Project Fund of Cumhuriyet University under project number V-011. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK | en_US |
dc.relation.isversionof | 10.3906/vet-1707-93 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Dirofilaria immitis | en_US |
dc.subject | dogs | en_US |
dc.subject | enzyme-linked immunosorbent assay | en_US |
dc.subject | microscopy | en_US |
dc.subject | polymerase chain reaction | en_US |
dc.subject | Turkey | en_US |
dc.title | Survey of Dirofilaria immitis in dogs from Sivas Province in the Central Anatolia Region of Turkey | en_US |
dc.type | article | en_US |
dc.relation.journal | TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES | en_US |
dc.contributor.department | [Atas, Ahmet Duran] Cumhuriyet Univ, Dept Parasitol, Fac Med, Sivas, Turkey -- [Altay, Kursat -- Ozkan, Erkan] Cumhuriyet Univ, Dept Parasitol, Fac Vet Med, Sivas, Turkey -- [Alim, Ahmet] Cumhuriyet Univ, Dept Microbiol, Fac Med, Sivas, Turkey | en_US |
dc.identifier.volume | 42 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.endpage | 134 | en_US |
dc.identifier.startpage | 130 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |