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dc.contributor.authorTemel, Yusuf
dc.contributor.authorKocyigit, Umit M.
dc.date.accessioned2019-07-27T12:10:23Z
dc.date.accessioned2019-07-28T09:40:33Z
dc.date.available2019-07-27T12:10:23Z
dc.date.available2019-07-28T09:40:33Z
dc.date.issued2017
dc.identifier.issn1095-6670
dc.identifier.issn1099-0461
dc.identifier.urihttps://dx.doi.org/10.1002/jbt.21927
dc.identifier.urihttps://hdl.handle.net/20.500.12418/6695
dc.descriptionWOS: 000408915800002en_US
dc.descriptionPubMed ID: 28557068en_US
dc.description.abstractGlucose-6-phosphate dehydrogenase (G6PD) is the first enzyme on which the pentose phosphate pathway was checked. In this study, purification of a G6PD enzyme was carried out by using rat erythrocytes with a specific activity of 13.7EU/mg and a yield of 67.7 and 155.6-fold by using 2,5-ADP Sepharose-4B affinity column chromatography. For the purpose of identifying the purity of enzyme and molecular mass of the subunit, a sodium dodecyl sulfate-polyacrylamide gel electrophoresis was carried out. The molecular mass of subunit was calculated 56.5kDa approximately. Then, an investigation was carried out regarding the inhibitory effects caused by various metal ions (Fe2+, Pb2+, Cd2+, Ag+, and Zn2+) on G6PD enzyme activities, as per Beutler method at 340nm under in vitro conditions. Lineweaver-Burk diagrams were used for estimation of the IC50 and K-i values for the metals. K-i values for Pb+2, Cd+2, Ag+, and Zn+2 were 113.3, 215.2, 19.4, and 474.7M, respectively.en_US
dc.language.isoengen_US
dc.publisherWILEYen_US
dc.relation.isversionof10.1002/jbt.21927en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectenzymeen_US
dc.subjecterythrocyteen_US
dc.subjectglucose-6-phosphate dehydrogenase (G6PD)en_US
dc.subjectheavy metalen_US
dc.subjectpurificationen_US
dc.subjectraten_US
dc.titlePurification of glucose-6-phosphate dehydrogenase from <bold>rat</bold> (Rattus norvegicus) <bold>erythrocytes</bold> and inhibition effects of some metal ions on enzyme activityen_US
dc.typearticleen_US
dc.relation.journalJOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGYen_US
dc.contributor.department[Temel, Yusuf] Bingol Univ, Solhan Vocat Sch Hlth Serv, TR-12000 Bingol, Turkey -- [Kocyigit, Umit M.] Cumhuriyet Univ, Vocat Sch Hlth Serv, TR-58140 Sivas, Turkeyen_US
dc.identifier.volume31en_US
dc.identifier.issue9en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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