dc.contributor.author | Ozkan, Mehtap Tugce | |
dc.contributor.author | Aliyazicioglu, Rezzan | |
dc.contributor.author | Demir, Selim | |
dc.contributor.author | Misir, Sema | |
dc.contributor.author | Turan, Ibrahim | |
dc.contributor.author | Yildirmis, Sermet | |
dc.contributor.author | Aliyazicioglu, Yuksel | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:44:00Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:44:00Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1735-7780 | |
dc.identifier.issn | 2228-7876 | |
dc.identifier.uri | https://dx.doi.org/10.5812/jjnpp.40073 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/6851 | |
dc.description | WOS: 000419309900011 | en_US |
dc.description.abstract | Background: Primula vulgaris has been used in traditional treatment, and its biological functions are attributed to its polyphenolics content. Objectives: The aim of this study was to determine the phenolics composition and the antioxidant activity of water extract of P. vulgaris (WEP) and to determine its probable preventive effects against H2O2-induced DNA damage in human fibroblast cells. Methods: The total polyphenolic content (TPC), ferric reducing antioxidant power (FRAP), and radical scavenging activity of WEP were determined using spectrophotometric methods. Phenolic compounds and antigenotoxic effects of WEP were evaluated using HPLC and comet assay, respectively. Results: The TPC and FRAP values of WEP were 15.023 +/- 0.84 mg gallic acid and 82.63 +/- 0.31 mu M trolox per g sample, respectively. rho-coumaric acid and rutin were detected as major phenolics. Moreover, WEP reduced H2O2-induced DNA damage in a concentration dependent manner in fibroblast cells compared to the positive controls (only 20 mu M H2O2 treatment). Conclusions: Primula vulgaris can be used in food, cosmetics, and drug industries because of its antioxidant and antigenotoxic activities. | en_US |
dc.description.sponsorship | Foundation of Scientific Research of Karadeniz Technical University | en_US |
dc.description.sponsorship | The authors thank Dr. Huseyin Sahin (Giresun University) for his assistance with HPLC analysis. This work was supported by Foundation of Scientific Research of Karadeniz Technical University. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | KOWSAR PUBL | en_US |
dc.relation.isversionof | 10.5812/jjnpp.40073 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Antioxidant Effect | en_US |
dc.subject | Antigenotoxicity | en_US |
dc.subject | Comet Assay | en_US |
dc.subject | DNA Damage | en_US |
dc.subject | HPLC | en_US |
dc.subject | Primula vulgaris | en_US |
dc.title | Phenolic Characterisation and Antioxidant Activity of Primula vulgaris and Its Antigenotoxic Effect on Fibroblast Cells | en_US |
dc.type | article | en_US |
dc.relation.journal | JUNDISHAPUR JOURNAL OF NATURAL PHARMACEUTICAL PRODUCTS | en_US |
dc.contributor.department | [Ozkan, Mehtap Tugce -- Aliyazicioglu, Rezzan -- Yildirmis, Sermet] Karadeniz Tech Univ, Dept Biochem, Fac Pharm, TR-61080 Trabzon, Turkey -- [Demir, Selim] Karadeniz Tech Univ, Dept Nutr & Dietet, Fac Hlth Sci, TR-61080 Trabzon, Turkey -- [Misir, Sema] Karadeniz Tech Univ, Inst Hlth Sci, Dept Med Biochem, TR-61080 Trabzon, Turkey -- [Misir, Sema] Cumhuriyet Univ, Dept Biochem, Fac Pharm, TR-58140 Sivas, Turkey -- [Turan, Ibrahim] Gumushane Univ, Fac Engn & Nat Sci, Dept Genet & Bioengn, TR-29100 Gumushane, Turkey -- [Turan, Ibrahim] Gumushane Univ, Med Plants Tradit Med Practice & Res Ctr, TR-29100 Gumushane, Turkey -- [Aliyazicioglu, Yuksel] Karadeniz Tech Univ, Dept Med Biochem, Fac Med, TR-61080 Trabzon, Turkey | en_US |
dc.contributor.authorID | Demir, Selim -- 0000-0002-1863-6280 | en_US |
dc.identifier.volume | 12 | en_US |
dc.identifier.issue | 1 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |