dc.contributor.author | Kalin, Recep | |
dc.contributor.author | Karahan, Murat | |
dc.contributor.author | Acik, Mehmet Nuri | |
dc.contributor.author | Tasdemir, Bulent | |
dc.contributor.author | Cetinkaya, Burhan | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:44:12Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:44:12Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1300-6045 | |
dc.identifier.uri | https://dx.doi.org/10.9775/kvfd.2017.17995 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/6966 | |
dc.description | WOS: 000412413200010 | en_US |
dc.description.abstract | The aim of this study was to evaluate a simple and rapid DNA extraction method combined with a multiplex polymerase chain reaction (mPCR) for the identification of the major mastitis pathogens (Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli and Mycoplasma bovis) from milk samples. Of the 200 California Mastitis Test (CMT) positive milk samples, 45 (22.5%), 21 (10.5%) and 11 (5.5%) were detected as positive for the presence of S. aureus, S. agalactiae and E. coli by culture, respectively. In mPCR by DNA isolation method optimised here, S. aureus, S. agalactiae and E. coli were detected in 26.5% (53/200), 12% (24/200) and 6% (12/200) of the milk samples, respectively. The abovementioned agents were observed in similar proportions when the samples were analysed by a commercial DNA isolation kit. On the other hand, M. bovis was not detected in any of the milk samples by either culture or mPCR methods. A significant difference was determined between the results of culture and mPCRs (P< 0.001). Diagnostic sensitivity and specificity of the optimised mPCR were calculated as 100% and 89.2% respectively, when culture results were considered as reference. The results suggest that the mPCR assay employed in this study could be used as an alternative routine diagnostic method for rapid, sensitive, and specific simultaneous detection of major mastitis agents in bovine milk samples. | en_US |
dc.description.sponsorship | General Directorate of Agricultural Research and Policy [TAGEM/HS/01/02/08/129] | en_US |
dc.description.sponsorship | We wish to thank Osman KOC and personnel at Elazig Veterinary Control Institute, Turkey for their contributions to this work, which was funded by the General Directorate of Agricultural Research and Policy (TAGEM/HS/01/02/08/129). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | KAFKAS UNIV, VETERINER FAKULTESI DERGISI | en_US |
dc.relation.isversionof | 10.9775/kvfd.2017.17995 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Mastitis | en_US |
dc.subject | Major Pathogens | en_US |
dc.subject | DNA isolation | en_US |
dc.subject | Multiplex PCR | en_US |
dc.title | Development of A Multiplex PCR Method for Direct Detection of Common Mastitis Pathogens in Bovine Milk Samples | en_US |
dc.type | article | en_US |
dc.relation.journal | KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI | en_US |
dc.contributor.department | [Kalin, Recep -- Karahan, Murat] Cumhuriyet Univ, Fac Vet Med, Dept Microbiol, TR-58140 Sivas, Turkey -- [Karahan, Murat] Kyrgyz Turkish Manas Univ, Fac Vet Med, KG-720044 Bishkek, Kyrgyzstan -- [Acik, Mehmet Nuri] Bingol Univ, Fac Vet Med, Dept Microbiol, TR-12000 Bingol, Turkey -- [Tasdemir, Bulent] Vet Control Inst, TR-23200 Elazig, Turkey -- [Cetinkaya, Burhan] Firat Univ, Fac Vet Med, Dept Microbiol, TR-2311 Elazig, Turkey | en_US |
dc.contributor.authorID | KALIN, Recep -- 0000-0002-9173-9550 | en_US |
dc.identifier.volume | 23 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.endpage | 931 | en_US |
dc.identifier.startpage | 925 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |