dc.contributor.author | Cevik, Ozge | |
dc.contributor.author | Adiguzel, Zelal | |
dc.contributor.author | Baykal, Ahmet Tarik | |
dc.contributor.author | Sener, Azize | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:44:22Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:44:22Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1300-0152 | |
dc.identifier.issn | 1303-6092 | |
dc.identifier.uri | https://dx.doi.org/10.3906/biy-1509-64 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/7042 | |
dc.description | WOS: 000394539800004 | en_US |
dc.description.abstract | Platelets are sensitive cells and are easily activated by different stimulants in the circulation system. It is known that tumor necrosis factor-alpha (TNF-alpha) is a proinflammatory cytokine and plays a role in inflammation. The role of TNF-a in the apoptotic process in blood platelets is unknown. In order to study the formation of apoptosis in platelets after incubation with TNF-alpha and/or ADP, several biomarkers were chosen: phosphatidylserine (PS) exposure and P-selectin binding; cGMP, Cyt-c, and Ca2+ levels and NOS activation; and gene and protein expression of caspase-3 and iNOS. Platelets were incubated with 100 pg/mL TNF-a and/or 50 mM iNOS specific inhibitor, such as at 1400 W for 1 h at 37 degrees C in the presence of 5 mu M ADP. According to the results, the levels of PS exposure and P-selectin binding were significantly higher in TNF-alpha + ADP platelets, which decreased with the addition of 1400 W. A significant induction in cGMP, Cyt-c, and Ca2+ levels was observed in platelets treated with TNF-alpha + ADP. On the other hand, the upregulation of the apoptotic gene caspase-3 and iNOS expression levels in TNF-alpha-treated and ADP-activated platelets was significantly reversed with the addition of 1400 W. These data demonstrate that TNF-alpha promotes iNOS expression through caspase-3 stimulation in human platelets, and its concomitance leads to the triggering of apoptosis-mediated inflammation upon platelet activation. | en_US |
dc.description.sponsorship | Marmara University Research Unit [SAG-C-DRP-010710-0216]; Cumhuriyet University Research Unit [ECZ-001] | en_US |
dc.description.sponsorship | The research was supported by the Marmara University Research Unit (Grant No. SAG-C-DRP-010710-0216) and was partly supported by the Cumhuriyet University Research Unit (Grant No. ECZ-001). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY | en_US |
dc.relation.isversionof | 10.3906/biy-1509-64 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Platelets | en_US |
dc.subject | apoptosis | en_US |
dc.subject | TNF-alpha | en_US |
dc.subject | iNOS | en_US |
dc.subject | caspase-3 | en_US |
dc.title | Tumor necrosis factor-alpha induced caspase-3 activation-related iNOS gene expression in ADP-activated platelets | en_US |
dc.type | article | en_US |
dc.relation.journal | TURKISH JOURNAL OF BIOLOGY | en_US |
dc.contributor.department | [Cevik, Ozge] Cumhuriyet Univ, Dept Biochem, Fac Pharm, Sivas, Turkey -- [Cevik, Ozge -- Sener, Azize] Marmara Univ, Dept Biochem, Fac Pharm, Istanbul, Turkey -- [Adiguzel, Zelal] TUBITAK Marmara Res Ctr, Genet Engn & Biotechnol Inst GEBI, Kocaeli, Turkey -- [Baykal, Ahmet Tarik] Acibadem Univ, Sch Med, Dept Biochem, Istanbul, Turkey | en_US |
dc.contributor.authorID | Baykal, Ahmet -- 0000-0002-8814-7351; Cevik, Ozge -- 0000-0002-9325-3757 | en_US |
dc.identifier.volume | 41 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.endpage | 40 | en_US |
dc.identifier.startpage | 31 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |