dc.contributor.author | Cetinkaya, Serap | |
dc.contributor.author | Akkaya, Birnur | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:44:38Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:44:38Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0928-4931 | |
dc.identifier.issn | 1873-0191 | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.msec.2016.06.040 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/7126 | |
dc.description | WOS: 000382600000068 | en_US |
dc.description | PubMed ID: 27524056 | en_US |
dc.description.abstract | In this study, newly modified hydroxyapatite microbeads were used to isolate alpha-lactalbumin (alpha-LA) from cow's milk by a single step. Inorganic material, pure hydroxyapatite was embedded in ethylene glycol dimethacrylate (EGDMA) in the presence of pore maker. Newly synthesized hydroxyapatite microbeads were used firstly for adsorption of alpha-LA from aqueous solution. The maximum adsorption capacity of alpha-LA from aqueous solution was observed at pH 7.0 (10.0 mM phosphate buffer) at 25 degrees C in a batch system, in which parallel separate experiments were performed independently. Synthesized hydroxyapatite microbeads and pure hydroxyapatite were used for adsorption studies from whole milk (without pre-treatment such as fat and caseins removal). Our method is cheap, simple, efficient and rapid. The results showed that modified hydroxyapatite microbeads had selective affinity to alpha-LA because of good adsorption properties. (C) 2016 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | Cumhuriyet University (CUBAP) [F-421] | en_US |
dc.description.sponsorship | This work was supported by The Research Fund of Cumhuriyet University (CUBAP, project number F-421). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.relation.isversionof | 10.1016/j.msec.2016.06.040 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Alpha-lactalbumin | en_US |
dc.subject | Hydroxyapatite | en_US |
dc.subject | Milk proteins | en_US |
dc.subject | Selective adsorption | en_US |
dc.subject | Polymerization | en_US |
dc.title | Selective and single step adsorption of a-lactalbumin from whole cow's milk on hydroxyapatite microbeads | en_US |
dc.type | article | en_US |
dc.relation.journal | MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | en_US |
dc.contributor.department | [Cetinkaya, Serap -- Akkaya, Birnur] Cumhuriyet Univ, Dept Mol Biol & Genet, Sivas, Turkey | en_US |
dc.identifier.volume | 68 | en_US |
dc.identifier.endpage | 578 | en_US |
dc.identifier.startpage | 573 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |