The thioredoxin reductase activity and hypoxia-inducible factor 1 alpha level in anaerobic and aerobic macrophages

Abstract

OBJECTIVES: This study was aimed to demonstrate the thioredoxin reductase (TrxR) activities and Hypoxia-Inducible Factor 1 alpha (HIF-1 alpha) levels in macrophage cell line incubated in aerobic and anaerobic settings. BACKGROUND: Pathological situations including inflammatory disorders are associated with the infiltration of phagocyte system cells into damaged tissues. Whenever the environment of tissues converts into hypoxic conditions, phagocytic cells develop an adaptive mechanism in order to fulfill their defense functions. MATERIALS AND METHODS: The macrophage cells were prepared as two replications both for aerobic and anaerobic media. The E. coli bacteria were inoculated onto the some macrophage culture mediums. TrxR and HIF-1 alpha levels of the samples, obtained from all growth cultures, were measured with the ELISA. RESULTS: On the 5th and 6th day, there was a continuous increase in the count of bacteria in the aerobic medium, while a continuous decrease in the count of bacteria in the anaerobic medium. The TrxRand the HIF-1 alpha levels in the groups with anaerobic and aerobic macrophages with or without E. coli bacteria were evaluated. A statistically significant difference was found between these groups in terms of TrxR and HIF-1 alpha levels (p < 0.05). CONCLUSIONS: Increased TrxR and HIF-1 alpha levels were thought to have an effect on the adaptation of the macrophages in the anaerobic environment (Tab. 5, Fig. 2, Ref. 23). Text in PDF www.elis.sk.