dc.contributor.author | Karakus, Gulderen | |
dc.contributor.author | Yaglioglu, Ayse Sahin | |
dc.contributor.author | Zengin, Haci Bayram | |
dc.contributor.author | Karakus, Nihat | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:56:28Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:56:28Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1309-0801 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/8020 | |
dc.description | WOS: 000361222600010 | en_US |
dc.description.abstract | Poly (maleic anhydride-co-vinyl acetate) (MAVA) copolymer was synthesized by free-radical-polymerization reaction in methyl ethyl ketone (MEK) at 80 degrees C using benzoyl-peroxide (BPO) as the radical-initiator. MAVA was then modified with anti-leukemic chemotherapy-agent cytosine beta-D-arabinofuranoside hydrochloride (CF). Modification was performed at 70 degrees C in dimethylformamide (DMF) containing triethylamine (Et3N) as the catalyst. Structural characterization of the copolymer and copolymer/drug couple (MAVA/CF) was carried out by Fourier Transform Infrared (FTIR) and Nuclear Magnetic Resonance (H-1-NMR). FTIR and H-1-NMR spectra confirmed the modification reaction. UV-Spectrophotometric measurements indicated that MAVA/CF kept its molecular integrity in physiological-body-fluid, PBS, for first four days. Antiproliferative activities of MAVA/CF were also determined by BrdU-cell-proliferation-ELISA assays using C6 and HeLa cell lines (cisplatin and 5-fluorouracil used as positive control). MAVA/CF appeared to have little antiproliferative activity against C6 cell line while samples didn't have antiproliferative activity against HeLa cell line at low concentrations (<100 mu g/ml). Reaction mechanism was also recommended for modification product MAVA/CF. | en_US |
dc.description.sponsorship | Sciences Research Projects Foundation of Cumhuriyet University [F258, F339] | en_US |
dc.description.sponsorship | This work was supported by the Sciences Research Projects Foundation of Cumhuriyet University (Project No: F258 and F339). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | MARMARA UNIV, FAC PHARMACY | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Antiproliferative activity | en_US |
dc.subject | copolymer modification | en_US |
dc.subject | cytosine beta-D-arabinofuranoside hydrochloride | en_US |
dc.subject | physiological body fluid PBS | en_US |
dc.subject | poly(maleic anhydride-co-vinyl acetate) | en_US |
dc.title | Synthesis, characterization and antiproliferative activities of novel modified poly (maleic anhydride-co-vinyl acetate)/cytosine beta-D-arabinofuranoside hydrochloride conjugate | en_US |
dc.type | article | en_US |
dc.relation.journal | MARMARA PHARMACEUTICAL JOURNAL | en_US |
dc.contributor.department | [Karakus, Gulderen] Cumhuriyet Univ, Fac Pharm, Dept Pharmaceut Chem, Sivas, Turkey -- [Yaglioglu, Ayse Sahin] Cankiri Karatekin Univ, Dept Chem, Fac Sci, Cankiri, Turkey -- [Zengin, Haci Bayram -- Karakus, Nihat] Cumhuriyet Univ, Dept Chem, Fac Sci, Sivas, Turkey | en_US |
dc.identifier.volume | 19 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.endpage | 81 | en_US |
dc.identifier.startpage | 73 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |