Simple and useful method for determination of inorganic selenium species in real samples based on UV-VIS spectroscopy in a micellar medium
Abstract
A preconcentration and determination methodology utilizing the cloud point phenomenon was developed for the determination of inorganic selenium species using UV-VIS spectrophotometry. The cloud point extraction method was based on the selective formation of a complex between selenium(IV) ions and 4,5-diamino-6-hydroxy-2-mercapto pyrimidine (DAHMP). Then, the formed stable complex was extracted to a nonionic surfactant phase of Triton X-114. After phase separation was carried out via decantation, the surfactant-rich phase was diluted with 20% ethanol solution and the absorbance signal was measured at 458 nm using an UV-VIS spectrophotometer. Under the optimized conditions, linear calibration curves were obtained over a range of 20-1500 ng mL(-1) with detection and quantification limits of 6.06 ng mL(-1) and 19.89 ng mL(-1), respectively. The relative standard deviation was 2.80% for 5 replicate measurements at 250 mu g L-1 concentration level. The proposed method is very versatile and economic as only a conventional spectrophotometer is used for the measurements. The method was validated by the analysis of two certified reference materials and a recovery test. Finally, the method was applied to the determination of the total Se amount in real samples including water samples and hen eggs containing selenium.
Source
ANALYTICAL METHODSVolume
7Issue
3Collections
- Makale Koleksiyonu [5200]
- Makale Koleksiyonu [5745]
- Öksüz Yayınlar Koleksiyonu - WoS [6162]