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Transfection of human kidney epithelial 293 cells with bacterial cytosine-5-methyltransferase M. Msp 1and the SV40 nuclear localisation sequence of VP1
(1999)
The aim of this study was to con-struct a plasmid that would direct the expres-sion of a bacterial DNA methyltransferase in cultured mammalian cells. It was carried out with a PCR-based strategy for introducing three ...
In vivo DNA methylation of escherichia coli DH5 and top 1OF'strains by bacterial cytosine-5 methyltransferase M. Msp1
(1998)
Kromatin düzeyinde metillenmis CpG dinükleotidleri, metil grubu tasımayan nükleotidlerin aksine bir modifiye restriksiyon enzimi olan R. Msp1 enzimine dirençlidirler. Yapısında metillenmis dinükleotid (mCpG) tasıyan bu ...
The construction of a mammalian tranfection vector for expression of cytosine-5 specific DNA methyltransferase gene M.Msp1 in cultured cells
(1998)
Ekspresyon vektörleri normal yada rekombine bir genin istenilen hücreye aktarılması, ekspresyonu ve proteinin saflastırılmasına olanak saglayacak sekilde olusturulurlar. Günümüzde mem hücrelerin transient yada stabil gen ...