dc.contributor.author | Altun, Ahmet | |
dc.contributor.author | Temiz, Tijen Kaya | |
dc.contributor.author | Balci, Ezgi | |
dc.contributor.author | Polat, Zubeyde Akin | |
dc.contributor.author | Turan, Mustafa | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:58:50Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:58:50Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1000-9604 | |
dc.identifier.issn | 1993-0631 | |
dc.identifier.uri | https://dx.doi.org/10.3978/j.issn.1000-9604.2013.10.10 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/8563 | |
dc.description | WOS: 000326852400015 | en_US |
dc.description | PubMed ID: 24255582 | en_US |
dc.description.abstract | Objective: To investigate the effects of E7080 and N-5-(1-iminoethyl)-L-ornithine dihydrochloride (L-NIO) on colorectal cancer alone and in combination. Methods: HT29 colorectal cancer cell line from Sap Institute was used. Real-time cell analysis (xCELLigence system) was performed to determine the effects of E7080 and L-NIO on colorectal cell proliferation. While apoptosis was determined with Annexin V staining, and the effect of agents on angiogenesis was determined with chorioallantoic membrane (CAM) model. Results: We found that E7080 has a strong antiproliferative effect with an half maximum inhibition of concentration (IC50) value of 5.60x10(-8) mol/L. Also it has been observed that E7080 showed antiangiogenic and apoptotic effects on HT29 colorectal cancer cells. Antiangiogenic scores of E7080 were 1.2, 1.0 and 0.6 for 100, 10 and 1 nmol/L E7080 concentrations, respectively. Furthermore, apoptosis has been detected in 71% of HT29 colorectal cancer cells after administration of 100 nmol/L E7080 which may indicate strong apoptotic effect. Meanwhile administration of L-NIO alone did not show any effect, but the combination of E7080 with L-NIO increased the antiproliferative, antiangiogenic and apoptotic effects of E7080. Conclusions: Results of this study indicate that E7080 may be a good choice in treatment of colorectal tumors. Furthermore the increased effects of E7080 when combined with L-NIO raise the possibility to use a lower dose of E7080 and therefore avoid/minimize the side effects observed with E7080. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | SPRINGER | en_US |
dc.relation.isversionof | 10.3978/j.issn.1000-9604.2013.10.10 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | E7080 | en_US |
dc.subject | N-5-(1-iminoethyl)-L-ornithine dihydrochloride (L-NIO) | en_US |
dc.subject | colorectal cancer | en_US |
dc.subject | xCELLigence system | en_US |
dc.subject | tyrosine kinase (TK) | en_US |
dc.title | Effects of tyrosine kinase inhibitor E7080 and eNOS inhibitor L-NIO on colorectal cancer alone and in combination | en_US |
dc.type | article | en_US |
dc.relation.journal | CHINESE JOURNAL OF CANCER RESEARCH | en_US |
dc.contributor.department | [Altun, Ahmet -- Temiz, Tijen Kaya -- Balci, Ezgi] Cumhuriyet Univ, Sch Med, Dept Pharmacol, TR-58140 Sivas, Turkey -- [Polat, Zubeyde Akin] Cumhuriyet Univ, Sch Med, Dept Parasitol, TR-58140 Sivas, Turkey -- [Turan, Mustafa] Cumhuriyet Univ, Sch Med, Dept Gen Surg, TR-58140 Sivas, Turkey | en_US |
dc.contributor.authorID | Altun, Ahmet -- 0000-0003-2056-8683 | en_US |
dc.identifier.volume | 25 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.endpage | 584 | en_US |
dc.identifier.startpage | 572 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |