dc.contributor.author | Karakus, Gulderen | |
dc.contributor.author | Polat, Zubeyde Akin | |
dc.contributor.author | Yaglioglu, Ayse Sahin | |
dc.contributor.author | Karahan, Mesut | |
dc.contributor.author | Yenidunya, Ali Fazil | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:59:29Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:59:29Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 0920-5063 | |
dc.identifier.issn | 1568-5624 | |
dc.identifier.uri | https://dx.doi.org/10.1080/09205063.2012.750209 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/8660 | |
dc.description | WOS: 000319496400007 | en_US |
dc.description | PubMed ID: 23713427 | en_US |
dc.description.abstract | Poly(maleic anhydride-co-styrene) (MAST) was synthesized by a free-radical polymerization reaction. A bioactive molecule, procainamide hydrochloride (PH), was then conjugated to MAST. The conjugation product was named as MAST/PH. Structural characterization of MAST and MAST/PH was carried out by Fourier Transform Infrared and Nuclear Magnetic Resonance spectroscopy. Their molecular weights were determined by size-exclusion chromatography. A mechanism was then suggested for the conjugation reaction. The results of the cytotoxicity assay, employing a mouse fibroblast cell line (L929), indicated that MAST/PH had no cytotoxicity at concentrations 62gmL(1) (p>0.05). Antiproliferative activities of MAST/PH and PH were determined by the BrdU cell proliferation ELISA assay, using C6 and HeLa cell lines. In the experiment, two anticancer chemotherapy drugs, cisplatin and 5-fluorouracil, were included as positive control. Antiproliferative activity results demonstrated that MAST/PH yielded the highest suppression profile (approximately 42%) at 20g/ml, while free PH exerted the same activity at 100g/ml. Interestingly, both MAST/PH and PH suppressed the proliferation of only one of the cell lines, C6 cells. Both cisplatin and 5-fluorouracil yielded approximately 60% antiproliferative activity on C6 cells at 20 and 100g/ml concentrations. Antiangiogenic capacity of both MAST and MAST/PH was also investigated by using the chicken chorioallantoic membrane assay. Results obtained indicated that while MAST/PH could be included into the category of good antiangiogenic substances, the activity score of MAST was within the weak category. | en_US |
dc.description.sponsorship | Sciences Research Projects Foundation of Cumhuriyet University [F258] | en_US |
dc.description.sponsorship | This work was supported by Sciences Research Projects Foundation of Cumhuriyet University (Project No: F258). The structural characterizations were carried out at Technology Research and Developing Centre, Erciyes University, Kayseri, Turkey. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | TAYLOR & FRANCIS LTD | en_US |
dc.relation.isversionof | 10.1080/09205063.2012.750209 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | antiangiogenic effects | en_US |
dc.subject | antiproliferative activity | en_US |
dc.subject | cytotoxicity | en_US |
dc.subject | poly(maleic anhydride-co-styrene) modification | en_US |
dc.subject | procainamide hydrochloride | en_US |
dc.title | Synthesis, characterization, and assessment of cytotoxic, antiproliferative, and antiangiogenic effects of a novel procainamide hydrochloride-poly(maleic anhydride-co-styrene) conjugate | en_US |
dc.type | article | en_US |
dc.relation.journal | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | en_US |
dc.contributor.department | [Karakus, Gulderen] Cumhuriyet Univ, Sch Med, CUTFAM RCCUSM, Res Ctr, TR-58140 Sivas, Turkey -- [Polat, Zubeyde Akin] Cumhuriyet Univ, Sch Med, Dept Parasitol, TR-58140 Sivas, Turkey -- [Yaglioglu, Ayse Sahin] Cankiri Karatekin Univ, Dept Chem, Fac Sci, TR-18100 Cankiri, Turkey -- [Karahan, Mesut] Uskudar Univ, Fac Engn & Nat Sci, Dept Bioengn, TR-34662 Uskudar Istanbul, Turkey -- [Yenidunya, Ali Fazil] Cumhuriyet Univ, Fac Sci, Dept Mol Biol & Genet, TR-58140 Sivas, Turkey | en_US |
dc.contributor.authorID | KARAKUS, Gulderen -- 0000-0003-2596-9208; Yenidunya, Ali Fazil -- 0000-0002-9886-977X; Karahan, Mesut -- 0000-0002-8971-678X | en_US |
dc.identifier.volume | 24 | en_US |
dc.identifier.issue | 10 | en_US |
dc.identifier.endpage | 1276 | en_US |
dc.identifier.startpage | 1260 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |