dc.contributor.author | Akkaya, Birnur | |
dc.contributor.author | Yavuz, Handan | |
dc.contributor.author | Candan, Ferda | |
dc.contributor.author | Denizli, Adil | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:03:28Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:03:28Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0021-8995 | |
dc.identifier.uri | https://dx.doi.org/10.1002/app.34443 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/9044 | |
dc.description | WOS: 000302902200023 | en_US |
dc.description.abstract | Concanavalin A (Con A) immobilized magnetic poly(glycidyl methacrylate) (mPGMA) beads in monosize and spherical for (1.62 mu m in diameter) were used for the purification of human immunoglobulin G (IgG) from human plasma. Con A was immobilized by covalent binding onto the mPGMA beads. The maximum IgG adsorption on the mPGMA-Con A beads was observed at pH 6.0. The nonspecific IgG adsorption onto the plain mPGMA beads was very low (0.22 mg/g). Scatchard analysis of the adsorption isotherm for IgG on mPGMA-Con A beads showed an affinity constant (Ka) of 1.39 x 105 M-1 and a theoretical maximum adsorption capacity of 109.1 mg/g. An apparent IgG adsorption capacity of 66.2 mg/g was observed under the experimental conditions. IgG adsorption capacity from human plasma was observed as 48.0 mg/g. The adsorption of human serum albumin from plasma was 2.0 mg/g. The total protein adsorption was determined to be 50.0 mg/g. IgG molecules could be repeatedly adsorbed and eluted with the mPGMA-Con A beads without noticeable loss in the IgG adsorption capacity. (c) 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012 | en_US |
dc.description.sponsorship | Cumhuriyet University, Scientific Research Projects Commission [F-233] | en_US |
dc.description.sponsorship | Contract grant sponsor: Cumhuriyet University, Scientific Research Projects Commission; contract grant number: Project Number F-233. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | WILEY-BLACKWELL | en_US |
dc.relation.isversionof | 10.1002/app.34443 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | magnetic beads | en_US |
dc.subject | separation techniques | en_US |
dc.subject | chromatography | en_US |
dc.subject | lectin affinity chromatography | en_US |
dc.subject | concanavalin A | en_US |
dc.subject | IgG | en_US |
dc.title | Concanavalin a immobilized magnetic poly(glycidyl methacrylate) beads for antibody purification | en_US |
dc.type | article | en_US |
dc.relation.journal | JOURNAL OF APPLIED POLYMER SCIENCE | en_US |
dc.contributor.department | [Yavuz, Handan -- Denizli, Adil] Hacettepe Univ, Dept Chem, TR-06532 Ankara, Turkey -- [Akkaya, Birnur -- Candan, Ferda] Cumhuriyet Univ, Dept Chem, Sivas, Turkey | en_US |
dc.identifier.volume | 125 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.endpage | 1874 | en_US |
dc.identifier.startpage | 1867 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |