dc.contributor.author | Perez-Burgos, R. | |
dc.contributor.author | Grzelak, E. M. | |
dc.contributor.author | Gokce, G. | |
dc.contributor.author | Saurina, J. | |
dc.contributor.author | Barbosa, J. | |
dc.contributor.author | Barron, D. | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:03:37Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:03:37Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 1570-0232 | |
dc.identifier.issn | 1873-376X | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.jchromb.2012.05.002 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/9097 | |
dc.description | WOS: 000306247300009 | en_US |
dc.description | PubMed ID: 22658467 | en_US |
dc.description.abstract | This work was focused on the comparison of two clean-up methods to be used for the simultaneous determination of seven cephalosporins in cow muscle. In particular, the performance of novel dispersive solid phase extraction (d-SPE) procedures based on QuEChERS methodologies was assessed and compared with conventional SPE. The separation and detection of the analytes using both methods was carried out by LC-MS/MS to reach enough sensitivity to be compatible with the detection of the maximum residue limits (MRL) of cephalosporins as regulated by EU directives. The optimization of the clean-up step relied on experimental design in order to find the most suitable conditions with a reduced number of assays. Besides, multi-objective responses were used to reach an overall compromise in the recovery of all analytes simultaneously. The validation of the two methods was done according to the Directive 2002/657/EC. Linearity, decision limit, detection capability, detection and quantification limits (4-50 mu kg(-1)), precision (RSD less than 15% except for PIR) and recoveries were determined and adequate results with comparable values using QuEChERS and SPE methodologies. LOQ were better for SPE method (0.1-10 mu g kg(-1)) but both methods show LOQ below MRL values. Precision was slightly better for the QuEChERS method, that also presents better recoveries, higher than 85% except for cephalexin. (C) 2012 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | Spanish Ministerio de Educacion y Ciencia [CTQ2010-19044/BQU] | en_US |
dc.description.sponsorship | We wish to acknowledge the financial support from the Spanish Ministerio de Educacion y Ciencia (Project CTQ2010-19044/BQU). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.relation.isversionof | 10.1016/j.jchromb.2012.05.002 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Cephalosporins | en_US |
dc.subject | Beef tissues | en_US |
dc.subject | QuEChERS | en_US |
dc.subject | SPE | en_US |
dc.subject | Experimental design | en_US |
dc.subject | LC-MS/MS | en_US |
dc.subject | Validation according 2002/657/EC | en_US |
dc.title | Quechers methodologies as an alternative to solid phase extraction (SPE) for the determination and characterization of residues of cephalosporins in beef muscle using LC-MS/MS | en_US |
dc.type | article | en_US |
dc.relation.journal | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | en_US |
dc.contributor.department | [Perez-Burgos, R. -- Saurina, J. -- Barbosa, J. -- Barron, D.] Univ Barcelona, Dept Analyt Chem, Fac Chem, E-08028 Barcelona, Spain -- [Grzelak, E. M.] Marie Curie Sklodowska Univ, Dept Chromatog Methods, PL-20031 Lublin, Poland -- [Gokce, G.] Cumhuriyet Univ, Dept Elementary Sci Educ, Fac Educ, TR-58140 Sivas, Turkey | en_US |
dc.contributor.authorID | Barron, Dolores -- 0000-0002-2032-2838; Grzelak, Edyta M -- 0000-0002-3033-2917; Barbosa, Jose -- 0000-0002-0982-2049 | en_US |
dc.identifier.volume | 899 | en_US |
dc.identifier.endpage | 65 | en_US |
dc.identifier.startpage | 57 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |