dc.contributor.author | Gorgun, Salih | |
dc.contributor.author | Akpinar, Mehmet Ali | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:03:38Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:03:38Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 1303-2712 | |
dc.identifier.uri | https://dx.doi.org/10.4194/1303-2712-v12_2_03 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/9100 | |
dc.description | WOS: 000309697800003 | en_US |
dc.description.abstract | In this study, a lipase from the liver of Cyprinus carpi Linnaeus (1758) living in Todurge Lake (Sivas) was purified with purification parameters of 90.38 mu mol/dk.mg protein specific activity and 75.50 fold purity. The purification procedure consisted of preparation of homogenate, precipitation with polyethylene glycol-6000 (PEG-6000) and chromatographic techniques including in Q sepharose, sephacryl S 200 HR and phenyl sepharose CL-4B, respectively. The purified lipase showed a single band with approximately a molecular weight of 74 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). pH and temperature optimums for the purified enzyme were determined as 8 and 37 degrees C using p-nitrophenyl butirate (p-NPB) as substrate. K-m and V-max values were found as 0.17 mM p-NPB and 2.6 mu mol/ml.dk, respectively. It was observed that surface active agents, such as sodium dodecyl sulfate (SDS), Triton X-100 and Nataurocholate act as inhibitor on lipase activity. | en_US |
dc.description.sponsorship | Research Fund of Cumhuriyet University (CUBAP) (Sivas, Turkiye) [F-243] | en_US |
dc.description.sponsorship | We thank to Research Fund of Cumhuriyet University (CUBAP) (Sivas, Turkiye) for supporting this research, which is a part of PhD Thesis study under the project number F-243. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | CENTRAL FISHERIES RESEARCH INST | en_US |
dc.relation.isversionof | 10.4194/1303-2712-v12_2_03 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Cyprinus carpio | en_US |
dc.subject | lipase activity | en_US |
dc.subject | Q sepharose | en_US |
dc.subject | sephacryl S 200 HR | en_US |
dc.subject | phenyl sepharose CL-4B | en_US |
dc.subject | Todurge Lake | en_US |
dc.title | Purification and Characterization of Lipase from the Liver of Carp, Cyprinus carpio L. (1758), Living in Lake Todurge (Sivas, Turkiye) | en_US |
dc.type | article | en_US |
dc.relation.journal | TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES | en_US |
dc.contributor.department | [Gorgun, Salih -- Akpinar, Mehmet Ali] Cumhuriyet Univ, Fac Sci, Dept Biol, TR-58140 Sivas, Turkey | en_US |
dc.identifier.volume | 12 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.endpage | 215 | en_US |
dc.identifier.startpage | 207 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |