dc.contributor.author | Akkaya, Birnur | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:03:58Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:03:58Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0927-7765 | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.colsurfb.2011.11.033 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/9203 | |
dc.description | WOS: 000300859000021 | en_US |
dc.description | PubMed ID: 22197734 | en_US |
dc.description.abstract | In this work, a new microcomposite composed of bentonite, acrylamide and histidine, as a pseudospecific ligand, was synthesized by bulk polymerization. The aim of this study was to improve IgG adsorption capacity of bentonite by incorporating histidine. The surface areas of the bentonite and bentonite-acrylamide-histidine microcomposites were 33.4 and 1.42 m(2)/g, respectively. The amount of histidine was found to be 50 mu mol/g bentonite via elemental analysis. Adsorption capacity was at the value of 100 mg/g from aqueous solution while adsorption capacity was 108 mg/g from human plasma with a purity of 90%. IgG biomolecules were able to be adsorbed and desorbed five times by using the same microcomposites without significant loss in their adsorption capacity. (C) 2011 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | Cumhuriyet University (CUBAP) [F-321] | en_US |
dc.description.sponsorship | This work was supported by The Research Fund of Cumhuriyet University (CUBAP, Project no: F-321). The author is also grateful to Dr. Recep Akkaya for providing valuable experimental studies. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.relation.isversionof | 10.1016/j.colsurfb.2011.11.033 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Acrylamide | en_US |
dc.subject | Affinity chromatography | en_US |
dc.subject | Bentonite | en_US |
dc.subject | Histidine | en_US |
dc.subject | Microcomposite | en_US |
dc.subject | IgG purification | en_US |
dc.title | IgG purification by bentonite-acrylamide-histidine microcomposite | en_US |
dc.type | article | en_US |
dc.relation.journal | COLLOIDS AND SURFACES B-BIOINTERFACES | en_US |
dc.contributor.department | Cumhuriyet Univ, Fac Sci, Dept Mol Biol & Genet, TR-58140 Sivas, Turkey | en_US |
dc.identifier.volume | 92 | en_US |
dc.identifier.endpage | 155 | en_US |
dc.identifier.startpage | 151 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |