dc.contributor.author | Akkaya, Birnur | |
dc.contributor.author | Akkaya, Recep | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:04:17Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:04:17Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0149-6395 | |
dc.identifier.issn | 1520-5754 | |
dc.identifier.uri | https://dx.doi.org/10.1080/01496395.2012.683124 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/9283 | |
dc.description | WOS: 000312984800009 | en_US |
dc.description.abstract | Among different adsorbents, a new support for immobilized metal affinity chromatography was used for lysozyme separation from egg white. For this purpose, acrylamide, bisacrylamide, bentonite, and L-histidine in the presence of an initiator (azobisisobutyronitrile, AIBN) were mixed and bentonite-acrylamide-histidine microcomposites were prepared by bulk polymerization. Characterization studies were performed by SEM, FT-IR, XRD, and BET. Specific surface area of the bentonite and BABH microcomposite was found to be 33.4 and 1.42 m2/g, respectively. Cu2+ ions (20 mu mol/g) were chelated on these bentonite-acrylamide-histidine (50 mu mol/g L-histidine incorporation) microcomposites. The lysozyme adsorption capacity of the Cu2+ ions chelated microcomposites increased the lysozyme adsorption up to 100 mg/g while the lysozyme adsorption capacity of the bentonite-acrylamide-histidine was 24 mg/g. More than 89% of the adsorbed lysozyme was desorbed in 1 h in the desorption medium containing 1.0 M NaCl. Bentonite-acrylamide-histidine-Cu(II) was used for the purification of lysozyme from chicken egg white. The purity of lysozyme was estimated by SDS-PAGE. Specific activity of the purified lysozyme was found as 50,000 U/mg using Micrococcus lysodeikticus as substrate. The new metal-chelate affinity microcomposites were suitable for repeated use for five cycles without a noticeable loss of capacity. | en_US |
dc.description.sponsorship | Research Fund of Cumhuriyet University | en_US |
dc.description.sponsorship | This work was supported by The Research Fund of Cumhuriyet University. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | TAYLOR & FRANCIS INC | en_US |
dc.relation.isversionof | 10.1080/01496395.2012.683124 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | egg white | en_US |
dc.subject | l-histidine | en_US |
dc.subject | lysozyme separation | en_US |
dc.subject | metal-chelate affinity chromatography | en_US |
dc.title | Cross-Linked Bentonite-Acrylamide-histidine-Based Metal-Chelate Affinity Microcomposites for Lysozyme Separation From Egg White | en_US |
dc.type | article | en_US |
dc.relation.journal | SEPARATION SCIENCE AND TECHNOLOGY | en_US |
dc.contributor.department | [Akkaya, Birnur] Cumhuriyet Univ, Fac Sci, Dept Mol Biol & Genet, TR-58140 Sivas, Turkey -- [Akkaya, Recep] Cumhuriyet Univ, Vocat Sch Hlth Serv, TR-58140 Sivas, Turkey | en_US |
dc.contributor.authorID | AKKAYA, Recep -- 0000-0002-3477-7198 | en_US |
dc.identifier.volume | 48 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.endpage | 280 | en_US |
dc.identifier.startpage | 272 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |