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    Effect of moderate electric field on structural and thermo-physical properties of sunflower protein and sodium caseinate
    (Elsevier Sci Ltd, 2021) Subasi, Busra Gultekin; Jahromi, Mastaneh; Casanova, Federico; Capanoglu, Esra; Ajalloueian, Fatemeh; Mohammadifar, Mohammad Amin
    Moderate electric field (MEF) was employed to sunflower protein isolate (SPI) and sodium caseinate (CAS) to change their structural and thermo-physical properties with a non-thermal approach. MEF treatment significantly reduced the average particle size with noticeable changes in the uniformity of SPI and CAS solutions. A maximum of 10% reduction in the surface tension was observed for 0.2% w/w SPI sample by applying 150 V for 20 s. This effect was less pronounced for CAS samples and for the protein concentration of 2% w/w. FTIR spectra provided an increased ?-helix content for SPI inducing a bulkier, loosened and more hydrophilic structure which is unlikely for CAS. Intrinsic Fluorescence Spectroscopy revealed that MEF treatments changed the tertiary structures of SPI and CAS. Denaturation temperatures and enthalpies of the SPI and CAS samples were decreased by MEF treatments.
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    Influence of moderate electric field on sodium caseinate structure and its techno-functionality
    (Elsevier, 2022) Jahromi, Mastaneh; Niakousari, Mehrdad; Golmakani, Mohammad Taghi; Subasi, Busra Gultekin; Mohammadifar, Mohammad Amin
    This study aimed to evaluate the effect of moderate electric field (MEF) treatment, as a non-thermal technology, on the structure and techno-functionality of sodium caseinate. Colloidal dispersions of caseinate were subjected to different MEF processing conditions (2.56 V cm(-1) for 1 and 2 h, 38.46 V cm(-1) for 5 and 20 s) and their structural, physical, emulsifying and film-forming characteristics were assessed. The Fourier transform infrared (FTIR) analysis of the caseinate showed higher beta-structures and a reduction in random coil conformation after MEF. The MEF-treated dispersions exhibited smaller particle size and developed emulsions with higher physical stability. The process increased the tensile strength (by 6.52-27.37%) and stretchability (by 12.17-54.30%) of caseinate film. A higher glass transition temperature in MEF-processed caseinate films was confirmed by modulated differential scanning calorimetry (MDSC) profiles. The scanning electron microscope (SEM) images showed a more uniform microstructure in treated caseinate films. The results suggest that MEF may be efficiently applied for mild modification of the structure of caseinate resulting in its property improvement.
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    Ultrasound Modification
    (Springer International Publishing, 2022) Jahromi, Mastaneh; Subasi, Büsra Gültekin
    Tremendous physicochemical and techno-functional activity of gums make them a good candidate to be widely applied in various fields including food, nutra/pharmaceutical, medical, cosmetics, and biosensors. Ultrasound technology is a promising method for green and non-thermal modification of gums and promotion of their techno-functionality. This chapter provides a detailed description and perspective of the ultrasound-assisted modification of gums and its impact on the structure, physicochemical, rheological, and interfacial performance of gums. The ultrasound-modified gums represent a great potential to be applied as new tuneable biopolymers for encapsulation of bioactive compounds, and design and development of delivery systems, emulsions, nanoparticles, and functional films and coatings. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2021.