Yazar "Karademir, Mustafa" seçeneğine göre listele

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    Assessment of the Effect of Low Back Pain on Life Satisfaction and Face-to-Face Communication Levels in Individuals
    (İstanbul Gelişim Üniversitesi, 2022) Karademir, Mustafa; Acar, Nihal
    Aim: This research aimed to explain the relationship between life satisfaction and face-to-face communication levels of people who have low back pain for a variety of reasons.Method: This study included a sample of 458 participants who had low back pain for any reason. The research was conducted using the exploratory research method, which describes the relationship between variables and expresses the study of topics that have been studied very little in the literature. 2 different scales and 4 different question forms were used in the research. The data obtained were tested by descriptive and parametric analyses with SPSS 17.0.Results: In the study, participants' daily functional status levels due to low back pain were found to be low. 65,2% of the sample was found to have chronic low back pain. It was found that the daily functional status of participants due to low back pain differed depending on socio-demographic variables such as age, marital status and education.Conclusion: In the study, a negative significant association was found between the daily functional status of participants due to low back pain and their life satisfaction and motivation for face-to-face communication
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    Borik asidin penisilin ile indüklenen deneysel epilepsi üzerine etkisi
    (Sivas Cumhuriyet University, 2019) Karademir, Mustafa; Arslan, Gökhan
    Amaç: Günlük hayatta insanlar, bir bor bileşiği olan borik aside maruz kalmaktadırlar. Bu çalışmada, düşük dozlarda uygulanan borik asidin penisilin kaynaklı epileptiform aktivite üzerindeki etkisini araştırdık. Ayrıca, borik asidin gabapentinin antikonvülsan etkisindeki rolünü de inceledik. Yöntem: Bu çalışmada, kırk sekiz adet erkek Wistar sıçanı kullanıldı. Üretan anestezisinden sonra, sıçanlar stereotaksi aletine bağlandı. Elektrokortikografi kayıtları için sol somatomotor korteks üzerine bir bipolar elektrot sabitlendi. İlk deney setinde, intrakortikal penisilin (500 IU) enjeksiyonundan 30 dakika sonra, dört farklı borik asit dozu (5, 10, 20 ve 40 mg/kg) intraperitoneal olarak uygulandı. İkinci deney setinde, borik asit (20 mg / kg), gabapentin (200 mg/kg) ve borik asit (20 mg/kg) + gabapentin (200 mg/kg) kombinasyonu uygulandı. İlaç enjeksiyonlarından sonra 120 dakika boyunca elektrokortikografi kayıtları kaydedildi ve sonrasında spike frekans ve amplitüdleri hesaplandı. Bulgular: 5 mg/kg dozunda uygulanan borik asit, kontrol grubuyla karşılaştırıldığında spike frekansını anlamlı ölçüde değiştirmedi. 10, 20 ve 40 mg / kg'lık dozlarında uygulanan borik asit ise, sırasıyla 50., 30. ve 30. dakikalarda ortalama spike frekansını artırarak prokonvülsan aktivite gösterdi. Gabapentin 30. dakikadan sonra ortalama spike frekansını azalttı. 20 mg/kg dozunda uygulanan borik asit, gabapentinin antikonvülsan etkisini inhibe etti. Spike amplüdü açısından hiçbir grup arasında anlamlı fark bulunmadı. Sonuç: Bu çalışma, borik asidin düşük dozlarının penisilin kaynaklı nöbetleri arttırdığını göstermektedir. Borik asit, bu prokonvulzan etkisini muhtemelen GABAerjik sistem üzerinden göstermektedir.
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    C2 transpedicular fixation technique in Hangman's Fracture
    (Sivas Cumhuriyet University, 2021) Küçükyıldız, Halil Can; Karademir, Mustafa; Güneş, Giray; Özüm, Ünal
    Objective : Traumatic spondylolisthesis of the axis, also known as hangman fractures, all cervical it accounts for 4-7% of all traumas and is the second most common axis after odontoid fractures. trauma. There are unstable fractures and the treatment options are immobilization with halo or surgery.is stabilization. By most authors, it is primarily associated with halo in patients with Type 1 and Type 2 fractures.Although external fixation and immobilization are recommended, halon is a difficult orthosis to use.Because of this, segmental motion sparing surgery provides rapid recovery in suitable patients may be an option. In the case we presented, the treatment approach in Hangman's fractures was reviewed in the literature.has been reviewed and discussed.Clinical Presentation: Our case, after AITC, with Levin Edwards Type 1A Hangman fracture, A 40-year-old female patient with no neurological deficits. The patient does not prefer to use a halo orthosis C2 transpedicular surgery was performed with a lag screw for motion-sparing surgery to a young patient fixation is planned.Technique: C2 transpedicular lag screw was applied to the patient with intraoperative fluoroscopy.Conclusion: C2 transpedicular fixation with Lag screw, according to posterior segmental stabilization a more minimally invasive and motion-sparing surgery, faster recovery time may be preferred to traditional posterior stabilization methods in selected patient groups due to The patient who did not prefer the use of orthoses due to the social disadvantages of immobilization with Halo it is a quick and cost-effective surgical option in groups with a rapid recovery time.
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    Effect of captopril on the oxidative damage caused by pentylenetetrazole in the SHSY-5Y human neuroblastoma cell line
    (Sivas Cumhuriyet University, 2020) Doğan, Murat; Karademir, Mustafa
    Objective: Epilepsy is a crucial brain disorder that seizures could cause a neuronal loss in the hippocampus. Oxidative stress has an important role in the pathology of this way. The aim of this study was to investigate the neuroprotective effect of captopril, on pentylenetetrazole (PTZ) induced epileptic seizures in SH-SY5Y cell line. Method: In this XTT cell viability assay, captopril was performed in vitro SH-SY5Y cell culture to evaluate PTZ-induced neurotoxicity. Tissue TOS concentrations at the cell supernatants were quantified with the automated assay method. Hydrogen peroxide was used for the calibration of the assay.Results: When XTT cell viability results were evaluated, captopril did not affect neuronal viability in SH-SY5Y cell line. Moreover, captopril did not have significant effect on TOS levels (***P>0. 001)Conclusions: Results showed that, captopril did not have neuroprotective properties in SH-SY5Y cell line after PTZ-induced neurotoxicity.
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    Enhanced Anticancer Effect of Temozolomide through Synergistic Combination with Diltiazem in Neuroblastoma (SH-SY5Y) Cell Line
    (Sivas Cumhuriyet Üniversitesi, 2023) Karademir, Mustafa; Surardamar, Saime
    Objective: This study aimed to assess the enhanced anticancer effect of Temozolomide (TMZ) through a synergistic combination with Diltiazem (DTZ) in the neuroblastoma cell line (SH-SY5Y). Material and Methods: The SH-SY5Y neuroblastoma cell line was cultured in a DMEM medium. TMZ and DTZ stock solutions were prepared and diluted to obtain different concentrations. The XTT assay was performed to evaluate cell viability. Cells were treated with TMZ alone or with DTZ for 24 and 48 hours. Viability was measured using the XTT-formazan product, and statistical analysis was performed. Results: The viability of neuroblastoma cells treated with TMZ alone and TMZ+DTZ combination was assessed. At 24 hours, TMZ showed no cytotoxic effect, while DTZ showed a low cytotoxic impact. At 48 hours, TMZ and TMZ+DTZ combination exhibited significant cytotoxic effects. The cytotoxic effects were concentration-dependent and time-dependent. DTZ alone showed significant cytotoxic effects on neuroblastoma cells at specific concentrations. Conclusion: The study demonstrated that TMZ alone and combined with DTZ had a significant cytotoxic effect on neuroblastoma cells. The combination of TMZ and DTZ may enhance the anticancer effect of TMZ and overcome drug resistance in neuroblastoma treatment. DTZ, a P-glycoprotein inhibitor, could prevent the efflux of TMZ from cancer cells, increasing its concentration and effectiveness.
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    Evaluation of Medical Specialization Education Students' Perceptions of Hospital Education Environment and Affecting Factors
    (Sivas Cumhuriyet University, 2022) Karademir, Mustafa; Ağadayı, Ezgi; Karahan, Seher; Karademir, Dilay
    Aim: Our aim in this study is to determine the perceptions of medical residency students studying at a university about the hospital education environment and the factors affecting it. Methods: This was a descriptive study. The population of the research consisted of specialty students studying at Sivas Cumhuriyet University. The research questionnaire was sent to the participants via text message to their personal mobile phone numbers. Informed consent was obtained from the participants on the first page of the survey link sent to them. The data collection tool used in the study included a total of 71 questions. The first part was created by the researchers through literature review and included 31 questions. The other 40 questions were Postgraduate Hospital Educational Environment Measure (PHEEM). Results: 266 of the 387 residency students in the faculty participated in the survey (Response rate: 68.7%). 79% of residency students studying in medical sciences and 56% in surgical sciences participated in the research. 11.7% (n=23) of residents from medical sciences and 28.6% (n=20) of residents from surgical sciences stated that no seminar hours were held. Postgraduate Hospital Educational Environment Measure mean score of the residency students was found to be 83.6 ± 25.5. The mean score did not differ significantly between departments (p=0.578). Those who thought that there were enough residents and faculty members had higher educational environment measure scores (respectively p=0.010 and p
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    Evaluation of potential cytotoxic and apoptotic effects of paclitaxel and docetaxel in human Burkitt lymphoma cell lines
    (Pharmacotherapy Group, 2022) Altun, Ahmet; Karademir, Mustafa; Altun, Guelsah
    Purpose: To study the cytotoxicity of paclitaxel and docetaxel on cell viability and apoptosis in Burkitt lymphoma cell lines.Methods: The cytotoxic activity of Paclitaxel and Docetaxel against Burkitt lymphoma cells was evaluated using the XTT cell viability test. The cells were exposed to increasing concentrations of paclitaxel and docetaxel ranging from 0.0001 to 10 mu M for 48 h. Cell cycle analysis and apoptosis were also assessed using flow cytometry-based experiments.Results: Both paclitaxel and docetaxel exhibited a concentration-dependent cytotoxic effect with IC50 of 5.32 and 6.58 mu M, respectively. Furthermore, paclitaxel and docetaxel were shown to have a significant apoptotic effect on Burkitt lymphoma cells (p < 0.01). Furthermore, paclitaxel and docetaxel arrested Burkitt lymphoma cells at the G2/M phase.Conclusion: Paclitaxel and docetaxel are potential candidates for the treatment of Burkitt lymphoma. Since both drugs are already licensed for many indications, the addition of Burkitt lymphoma to the list of indications of these drugs would be a much easier and faster process. However, there is a need for further in vitro and in vivo research to fully elucidate their mechanisms of action.
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    Evaluation of sagittal balance and spinopelvic parameters in patients with lumbar disc herniation
    (2018) Baran, Esin; Karademir, Mustafa
    Objective: The objective of this study was to analyze sagittal balance throughout with spinopelvic parameters, inpatients with a lumbar disc herniation and evaluate these patients with functional quality assessment questionnaires.Method: A total of 88 adult patients were evaluated in this study. All these patients were examined clinically in aphysical medicine and rehabilitation policlinic, from one physician in Sivas Numune State Hospital for a lumbar discherniation between 01 March-01 November 2018. The physical examination findings and records in the patient's fileswere evaluated. The Oswestry Disability Questionnaire (ODQ), Roland Morris Questionnaire (RDQ) and HealthAssessment Questionnaire (HAQ) were evaluated for all voluntary patients. All patients were confirmed with full spinalanteroposterior and lateral radiographs and lumbar MRI. Three group pathologies were evaluated in this study: Group1;disc herniation with bulging, Group 2; one level disc herniation and Group 3 multilevel disc herniation, includinglumbar disc protrusion, extrusion, and sequestration. Spino-pelvic parameters; lumbar lordosis (LL), pelvic incidence(PI), pelvic tilt (PT) and sacral slope (SS) were measured on full spine radiographs in a standing position.Results: LL was significantly less by multilevel disc herniation group as compared with bulging group and one leveldisc herniation group (p<0.05). It was a poor correlation between LL and waist circumference by multilevel discherniation group. When compared groups with functional assessment questionnaires, all groups were not statisticallydifferent each from other. We demonstrated not significantly different by all three groups between ODI, HAQ and RDIscores.Conclusions: The spinal sagittal imbalance caused by LDH is one type of compensatory response to prevent low backpain. This mechanism mainly includes a loss of LL and PT. Two important factors that need to be considered are theparavertebral muscles strengthening to prevent sagittal imbalance and keeping the body weight and waist circumferencein the normal range.
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    Evaluation of the vasorelaxant effect of Sugammadex on the arterial smooth muscle in rats
    (2023) Karademir, Mustafa; Altun, Gulsah; Karademir, Dilay
    Although NMBAs (Neuro Muscular Blocking Agents) have been used for a long time, postoperative residual curarization is still a significant problem. Nowadays, an agent named Sugammadex is used for the reversal of curarization. It has been presented as a safer agent than its predecessor, neostigmine. The main purpose of this study is to investigate the effects of Sugammadex on rat thoracic aorta and enlighten the mechanism of action and potential benefits in surgical operations with general anesthesia. Twenty Wistar albino rats were used for the experiments. Thoracic aorta segments have been removed and mounted to the organ bath. Contraction and relaxation responses were presented as a percentage of phenylephrine (3x10-5 M) contraction. After recording contractile responses of Sugammadex (10-8-10-4 M), relaxation responses of Sugammadex (10-8-10-4 M) have been recorded both in the presence and absence of L-NAME (3x10-5 M) (Potent Nitric Oxide Sentase Inhibitor). Finally, relaxation responses of sugammadex-rocuronium have been recorded. Sugammadex caused slightly noticeable and concentration- dependent contraction on isolated thoracic aorta strips. Sugammadex also caused potent and concentration-dependent relaxation on isolated rat thoracic aorta. The relaxation response caused by Sugammadex has been diminished significantly in the presence of L-NAME. Administration of rocuronium with Sugammadex did cause neither relaxation nor any additional contractile effect on isolated rat thoracic aorta strips. Sugammadex is a promising agent in reversing rocuronium- induced neuromuscular block. Although the adverse effects of this agent are not studied in detail, it seems Sugammadex is safer than neostigmine. The side effects of anesthetic agents are one of the main problems of surgical procedures, including neurosurgery, gynecology, and obstetrics. Especially acute hypotension may be fatal in neurosurgery and gynecological operations. Sugammadex should be used carefully in adjusted and individualized doses to avoid hypotension-related adverse effects.
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    Excessive numbers of students in Medical Faculties, Analysis of Cumhuriyet University
    (2018) Karademir, Mustafa; Karagöz, Naim; Altun, Ahmet; Çetin, İlhan
    Medical education is one of the oldest and toughest trainings in the world. Regardless of the fact that it has ancientnature and serious stacking of experience in the medical education field, it is still a very dynamic process andincorporates unique difficulties in its presence. Like the other professional training, it is essential for the student to learnby observing and experiencing from the tutor directly. In this context, the balance between the numbers of tutor and thestudent is essential. In recent years, the number of medical faculties and the number of students increased dramaticallyall over the country, and the balance is disrupted significantly. In the light of this knowledge, we conducted a year basedextensive analysis by using student and academic stuff records held by Rector's and Deans' offices to determine optimalstudent number of Sivas Cumhuriyet University. As a result, we managed to show that the numbers of medical studentsin Sivas Cumhuriyet University are over its capacity regarding academic stuff and infrastructure possibilities to give aproper education.
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    Executive Functions, Intellectual Capacity, and Psychiatric Disorders in Adults with Type 1 Chiari Malformation
    (Elsevier Science Inc, 2022) Yilmaz, Yavuz; Karademir, Mustafa; Caygin, Tulay; Yagcioglu, Oguz Kaan; Ozum, Unal; Kugu, Nesim
    OBJECTIVE: Contrary to popular belief, the cerebellum is involved not only in motor planning, balance, and coordination but also in cognitive processes. The present study aimed to investigate executive functions (EFs), intellectual capacity, and psychiatric disorders in adults with type 1 Chiari malformation, which is defined as a hindbrain anomaly that involves the cerebellum. METHODS: The study included 62 adults, with 29 in the CM group and 33 in the control group. EFs were evaluated using the Stroop test, number sequence learning test, and standardized mini-mental state examination (SMMSE). The intelligence quotient (IQ) was measured using the Kent EGY and Porteus maze tests, and psychiatric disorders were evaluated using the Structured Clinical Interview for DSM-5, Clinician Version (DSM-5-CV). RESULTS: The CM group took longer than the control to complete the Stroop test for each section (P < 0.005). Although the mean IQ scores of both groups were within the normal range, the CM group had a lower mean IQ score than the control group (P < 0.005). Although the mean SMMSE scores of both groups were within the normal range, the CM group had a lower mean SMMSE score than the control group (P < 0.005). The CM group had a higher rate of psychiatric comorbidities than the control group (P < 0.005). CONCLUSIONS: The study found that subjects with type 1 Chiari malformation performed worse in EFs than healthy controls and had a higher prevalence of psychiatric disorders.
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    GEBELİKTE EPİLEPSİ VE ANTİEPİLEPTİK TEDAVİNİN ANNE VE YENİDOĞAN ÜZERİNE ETKİSİ
    (T.C. Sağlık Bakanlığı Ankara Şehir Hastanesi, 2023) Karademir, Dilay; Karademir, Mustafa; Küçükyıldız, İrem; Yurtçu, Nazan; Can, İlkin Seda; Gültürk, Esra
    Amaç: Bu çalışmada amaç epilepsi tanısı almış ve antiepileptik ilaç kullanan gebelerde epilepsi hastalığının ve uygulanan antiepileptik tedavi protokolünün, anne, fetüs ve yenidoğan üzerine etkisinin değerlendirilmesidir. Gereç ve Yöntemler: Sivas Cumhuriyet Üniversitesi Tıp Fakültesi Kadın Hastalıkları ve Doğum Kliniği’nde 2015- 2020 tarihleri arasında gebelik takipleri yapılan ve epilepsi tanısı alan 55 gebe çalışmaya dahil edildi. Gebelerin yaşı, gebelik sayısı, doğum sayısı, epilepsi tanı yaşı, beden kitle indeksi (BKİ) , gebelikte sigara kullanımı, gebelik öncesi nöroloji kontrolüne gidip gitmedikleri, gebelik boyunca görülen nöbetlerin türü, hangi trimesterde nöbet geçirildiği ve sıklığı, gebelik sürecinde kullandıkları AEİ’lar ve tedavi protokolleri, gebelikte folik asit kullanımı değerlendirildi. Çalışmaya alınan gebelerde takipleri sırasında ortaya çıkan obstetrik komplikasyonlar ve doğum haftaları kaydedildi. Yenidoğan 1.-5. dk. Apgar skorları, fetal malformasyonlar ve fetal distres gibi erken neonatal sorunlar değerlendirildi. Bulgular: Yaşların ortanca değeri 26 (17-42) olarak bulunmuştur. Gebelik sayısının ortancası 2 (1-8)’dir. Doğum sayısının ortancası 1 (0-6)’dır. Çalışmaya alınan kadınların epilepsi tanı yaşlarının ortalaması ise 15 (3-29)’tir. Hastaların 34 (%61,9)’ü generalize, 2 (%3,6)’si parsiyel nöbet geçirmiştir. 19 (%34,5)’u ise gebelik sürecinde nöbet geçirmemiştir. Gebelik takibi boyunca komplikasyon gelişenlerin ortalaması % 43,6, gelişmeyenlerin ortalaması % 56,4 bulunmuştur. Hamilelikte trimesterlerde geçirilen nöbet sayısı ile 1. dakika Apgar skoru arasında istatistiksel farklılık vardır (p
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    Metabolomics in traumatic brain injuries
    (Nova Science Publishers, Inc., 2023) Karademir, Mustafa
    Traumatic brain injury (TBI) is an important pathology that can cause severe disability and unexpected sudden death. In the pathogenesis of TBI, many biochemical reactions develop at the cellular level and these mechanisms can cause significant changes in some of the body's metabolites. Clinical, radiological, and biochemical data are used in the evaluation of traumatic brain injuries due to the complex physiology of the brain. For the classification of trauma, clinicians often use the Glasgow Coma Scale (GCS). In addition, radiologically taken MR Spectroscopy evaluations and biochemical markers made from samples such as blood and cerebrospinal fluid provide enlightening information about TBI. Metabolomics can be biochemically detected in body fluids such as blood, cerebrospinal fluid, etc., in sudden traumatic brain injury and can give important information about the course of the disease and the severity of the trauma and the mortality and morbidity of the patient. © 2023 Nova Science Publishers, Inc. All rights reserved.
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    Neuroprotective effect of astaxanthin (ATX) against cognitive impairment on PTZ-induced epileptic seizures in rats and against PTZ-induced neurotoxicity in SH-SY5Y human neuroblastoma cell culture
    (Sivas Cumhuriyet University, 2019) Karademir, Mustafa; Gümüş, Erkan; Taştemur, Yaşar; Ergül, Merve; Ergül, Mustafa; Karabulut, Sebahattin; Akkaya, Recep
    Objective: Epilepsy is a common brain disorder that seizures could cause neuronal loss in the hippocampus. Oxidative stress has an important role in the pathology of this way. The aim of this study was to investigate the neuroprotective effect of astaxanthin (ATX), on pentylenetetrazole (PTZ) induced epileptic seizures in rats and in SH-SY5Y human neuroblastoma cell culture.Method: In our study, we used 42 male 230-250 g Wistar Albino rats. Animals were divided into seven groups as control, saline (PTZ; 1 ml/kg serum physiologic), positive control (2,5 mg/kg diazepam), 10 mg/kg, 20 mg/kg, 40 mg/kg and 80 mg/kg ATX for seven days. Thirty min after the administration of the last drug at the indicated doses, PTZ was administered 45 mg/kg to induce an epileptic seizure. The animals were observed for 30 min. Seizure stages according to the Racine Scale (RC) and first myoclonic jerk times (FMJ). Twenty four hours after PTZ injection, passive avoidance test was performed, and then brain tissues were removed for biochemical and histopathological evaluation. The hippocampal Cornu Ammonis 1 (CA1), CA3 and dentate gyrus (DG) regions were evaluated histopathologically regarding neuronal damage. Besides, oxidative stress markers total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI)) were measured in brain tissues. Furthermore, ATX was performed in vitro SH-SY5Y human neuroblastoma cell culture to evaluate PTZ-induced neurotoxicity.Results: When epileptic behaviors were evaluated, ATX did not affect RC and FMJ (p>0, 05). However, ATX reduced both cognitive impairment in passive avoidance test and neuronal damage in the hippocampus (p<0, 05). Moreover, ATX reduced both TOS levels and OSI in the brain (p<0, 05). Besides of these in vitro studies, ATX increased neuronal viability in vitro. Conclusions: Although ATX does not have antiepileptic properties directly, it has a protective effect on not only in vivo but also in vitro. These effects may occur by possible oxidative pathways.
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    Overcoming Drug Resistance: P-Gp Inhibitor Verapamil Enhances Temozolomide's Antiproliferative Effect in Neuroblastoma Cells
    (Maik Nauka/Interperiodica/Springer, 2024) Karademir, Mustafa; Altun, Ahmet
    Neuroblastoma is one of the most common and deadly childhood solid tumors. P-glycoprotein (P-gp) pump plays a role in developing resistance to many chemotherapeutic agents. The high expression of P-gp is associated with poor prognosis in drug resistance and neuroblastoma treatment. We aimed to evaluate the anticancer effect of Temozolomide (TMZ) on the SH-SY5Y human neuroblastoma cell line in the presence of P-gp inhibitor Verapamil (VER). In the present study, the antiproliferative effect of TMZ on SH-SY5Y cells alone and in combination with VER was evaluated using a colorimetric XTT viability test.SH-SY5Y cells were seeded to 96 well plates at 10 000 cells/well. TMZ (100 mu M-10 mM) and VER (0.25-10 mu g/mL) were applied to the cells alone first, then XTT measurements were performed after 24 and 48 h. In the study's second phase, VER was applied to the cells at the fixed concentration of 2.5 mu g/mL to block P-gp pumps, then increasing concentrations of TMZ were applied to the cells in the presence of VER. The current study showed that in addition to cytotoxic effects, VER + TMZ administration accelerated apoptosis in SH-SY5Y cells compared to TMZ utilization alone. The wound healing assay demonstrated that VER + TMZ combination also inhibited cell migration. Considering this evidence, combining TMZ with VER in the neuroblastoma cell line may inhibit migration and cell proliferation via the apoptosis pathway and produce a substantial anticancer effect.
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    PRP treatment for low back pain
    (Nova Science Publishers, Inc., 2024) Karademir, Mustafa
    Low back pain (LBP) is a common disorder that contributes significantly to social and economic loss. A significant portion of the population suffers from this disease, one of the most-seen musculoskeletal disorders. LBP can be associated with myofascial pain, facet joint pain, sacroiliac joint pain, discogenic pain, spinal stenosis, or failed back surgery. Platelet-rich plasma (PRP) is one of the novel treatment methods for LBP. PRP is plasma that contains concentrated platelets from autologous blood. Platelets contain cytokines and growth factors, thought to speed up the repair processes of the body. As a result, they may help manage inflamed nerves associated with lumbar neuropathic pain and canal stenosis. PRP is platelet sequestration in the plasma component of autologous blood that releases various intermediaries and growth factors. All these procedures ultimately result in tissue repair and LBP treatment. PRP is an efficient and less invasive method of treating LBP in routine clinical practice. © 2024 Nova Science Publishers, Inc. All rights reserved.
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    Unveiling the Protective Potential of Sugammadex against PTZ-Induced Epileptic Seizures in Mice: A Comprehensive Study on Oxidative Stress, Apoptosis, and Autophagy
    (Maik Nauka/Interperiodica/Springer, 2024) Karademir, Mustafa; Ozturk, Aysegul; Yulak, Fatih; Ozkaraca, Mustafa; Taskiran, Ahmet Sevki
    Sugammadex (SUG) is a modified gamma-cyclodextrin molecule used in patients under general anesthesia to reverse the effects of neuromuscular blocking agents. Besides, recent studies have shown that SUG positively affects the nervous system. However, its effect on seizures is still unclear. The current study aimed to examine the effects of SUG on pentylenetetrazole (PTZ)-induced epileptic seizures in mice. The mice were randomly divided into four groups. Group 1 was controlled, group 2 was administered saline (1 mL/kg serum physiologic), and Groups 3 and 4 were administered Sugammadex (150 and 300 mg/kg). Pentylenetetrazole (60 mg/kg) was given to induce seizures 30 min after saline or drug administration except for the control group. Total oxidant status (TOS) and total antioxidant status (TAS) levels in the hippocampus and cortex were measured using a commercial kit. 8-hydroxydeoxyguanosine (8-OHdG), 4-hydroxynonenal (4-HNE), 3,3 dityrosine, caspase-3, apoptosis-inducing factor (AIF), and light chain 3 (LC3B) levels in the hippocampal CA1 region and cortex after seizures were evaluated immunohistochemical staining. SUG reduced seizure stages and increased epileptic seizure onset times. Moreover, it decreased TOS levels and increased TAS levels in the hippocampus and cortex. Besides, after seizures, it reduced 4-HNE, 3,3 dityrosandine, caspase-3, and LC3B immunohistochemical scores in the hippocampal CA1 region and cortex. SUG has protective effects on pentylenetetrazole-induced seizures in mice, alleviating seizures, oxidative stress, apoptosis, and autophagy. The anticonvulsant mechanism of SUG may be related to the inhibition of the oxidative stress pathway.
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    Wi-Fi decreases melatonin protective effect and increases hippocampal neuronal damage in pentylenetetrazole induced model seizures in rats
    (MDPI, 2019) Akkaya, Recep; Gümüş, Erkan; Akkaya, Birnur; Karabulut, Sebahattin; Gülmez, Kader; Karademir, Mustafa; Taştemur, Yaşar
    Aim: Epilepsy is a common brain disorder in which the seizures could cause a neuronal loss in the hippocampus. Oxidative stress has an important role in the pathology of epilepsy. Some studies indicate that Wi-Fi increases oxidative stress and suppresses antioxidant systems. The aim of this study is to investigate the effect of Wi-Fi on melatonin anticonvulsive effect and oxidative damage in pentylenetetrazole-induced epileptic seizures in rats. Methods: In our study, we used 30 male Wistar Albino rats, 230?250 grams of the body weight. The animals were divided into five groups as control, saline (1 ml/kg/day olive oil for 30 days), Wi-Fi (12 h/day for 30 days), melatonin (10 mg/kg/day for 30 days) and melatonin + Wi-Fi (10 mg/kg/day +12 h/day for 30 days). In the thirtieth day, thirty minutes after the last drugs administration at the indicated doses, PTZ in 45 mg/kg was administered to induce epileptic seizure. The animals were observed for 30 min during the seizure stages (according to the Racine Scale) and first myoclonic jerk times (FMJ). Twenty-four hours after PTZ injection, brain tissues were removed for biochemical and histopathological evaluation. The hippocampal Cornu Ammonis (CA) 1, CA3 and DG (dentate gyrus) regions were histopathologically evaluated in terms of a neuronal damage in addition that oxidative stress markers (total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI)) were measured in brain tissues. Results: Wi-Fi was not found to affect behavioral changes associated with epilepsy (p > 0.05). However, Wi-Fi reduced anticonvulsive and antioxidant effect of melatonin (p < 0.05). Moreover, Wi-Fi increased neuronal damage in hippocampus (p < 0.05). Conclusion: Wi-Fi did not directly affect epileptic seizures. Nevertheless, it inhibits the positive effects of melatonin on epilepsy and it also has negative effects on hippocampal neuronal damage. These effects of Wi-Fi may occur via oxidative pathways. © 2019 Elsevier B.V.