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    Cytotoxicity evaluation of removable partial denture alloys were obtained from different laboratories
    (Cumhuriyet University Faculty of Dentistry, 2011) Sahin, Onur; Dogan, Derya Özdemir; Polat, Zübeyde Akin
    Objectives: The aim of this study was assess cytotoxicty of removable partial denture alloys which were obtained from different laboratories. Methods: 1 mm diameter, 1.5 mm thickness disc-shaped metal specimens which were applied sandblasting and sandblasting+polising process were obtained from four different laboratories activing in Sivas. Specimens were performed in sterile with ethylene oxide gas and then placed on to the L929 fibroblast cell culture. According to the 2009 ISO 10993-5 protochols, cytotoxicity were determined by means of agar overley test. Results: According to the lysis of the cells, specimens which were obtained from four laboratories, three of them were not cytotoxic while one of them was slightly cytotoxic. Moreover all groups in our study which applied sandblasting and sandblasting + polising process, were no significant difference in cytotoxicty. Conclusions: Laboratories use recasted metal alloys not only for economic reasons but also because of nesicence. The importance is emerging that technicians must be informed about the relationship between recasting process and biological risk and also size of the biological risk.
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    Observations on Acanthamoeba trophozoites in axenic cultures and their staining characteristics with different stains
    (2007) Polat, Zübeyde Akin; Ozçelik, Semra; Vural, Ayşe; Saygi, Gülendame
    Acanthamoeba spp. are among the most prevalent protozoa found in the environment. The species of this genus are the causative agents of granulomatous amebic encephalitis (GAE), a fatal disease of the central nervous system (CNS), and amebic keratitis (AK), a painful sight-threatening disease of the eye. In this study we have used two species of Acanthamoeba, Acanthamoeba castellanii and A. hatchetti, both were obtained from Vienna, Austria. They were cultivated on non-nutritious agar seeded with Escherichia coli and PPYG (protease peptone-yeast extract-glucose) medium. Our aim was to concentrate on three points in relation to the trophozoites and cysts stages of these species as follows: (i) to observe their morphology, (ii). to confirm our previous observation of a canal between two trophozoites. The bridge-like connection between these trophozoites greatly resembled the one that can be observed in conjugation during an exchange of genetic material. Two tro-phozoites with a bridge-like extension between them keep their position for at least 200 minutes. (iii). to detect the reactions of trophozoites to various stains. According to our findings in regard to these three points: (i). trophozoites with more than one nucleus are often seen in axenic cultures. (ii). This resembles a type of conjugation with a transfer of genetic material between two trophozoites. Certainly, this needs further investigation using more sophisticated methods. (iii). trophozoites equally stained well with Heidenhain's iron haematoxylin, Giemsa, PAS, Masson Trichrome, and Toludin-O stains. However, our results with reticulin, PAP, Van Gison, Musicarmine and Orsein stains were not satisfactory.