Cytotoxicity evaluation of removable partial denture alloys were obtained from different laboratories

Objectives: The aim of this study was assess cytotoxicty of removable partial denture alloys which were obtained from different laboratories. Methods: 1 mm diameter, 1.5 mm thickness disc-shaped metal specimens which were applied sandblasting and sandblasting+polising process were obtained from four different laboratories activing in Sivas. Specimens were performed in sterile with ethylene oxide gas and then placed on to the L929 fibroblast cell culture. According to the 2009 ISO 10993-5 protochols, cytotoxicity were determined by means of agar overley test. Results: According to the lysis of the cells, specimens which were obtained from four laboratories, three of them were not cytotoxic while one of them was slightly cytotoxic. Moreover all groups in our study which applied sandblasting and sandblasting + polising process, were no significant difference in cytotoxicty. Conclusions: Laboratories use recasted metal alloys not only for economic reasons but also because of nesicence. The importance is emerging that technicians must be informed about the relationship between recasting process and biological risk and also size of the biological risk.