Investigation of the Effect of Indatraline on Oxidative Damage Induced by Hydrogen Peroxide in C6 Glioma Cell Line

Basit Öğe Kaydı
dc.contributor.authorYulak, Fatih
dc.contributor.authorÜngür, Bünyamin
dc.date.accessioned2024-10-26T17:34:04Z
dc.date.available2024-10-26T17:34:04Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractOxidative stress is defined as an imbalance between the generation of reactive oxygen species (ROS) and their scavenging. Indatralin, which has serotonin reuptake inhibitory activity, has not yet been studied for its ability to prevent oxidative damage. Our research's objective was to find out how indatraline defends against oxidative damage. C6 cells were used in the study and four different cell groups were created. The control group received no therapy at all. For 24 hours, cells in the H2O2 group were exposed to 0.5 mM H2O2. The indatraline group received indatraline treatments for 24 hours at various doses (0.5, 1, 2.5, 5 and 10 ?M). For one hour, indatraline was administered to the indatraline + H2O2 group at various concentrations (0.5, 1, 2.5, 5 and 10 ?M) before the group was subjected to 0.5 mM H2O2 for 24 hours. Following the occurrence of oxidative damage, total antioxidant status (TAS) and total oxidant status (TOS) levels were determined. Cell viability was also evaluated using the XTT assay. As a result, after hydrogen peroxide-induced oxidative damage, indatraline at doses of 10, 5, and 2.5 ?M showed a protective effect by significantly enhanced cell survival in C6 cells(p < 0.001). Additionally, indatraline boosted the lowered TAS level while decreasing the elevated TOS levels following hydrogen peroxide-induced oxidative damage (p<0.001).
dc.identifier.doi10.17776/csj.1340869
dc.identifier.endpage649
dc.identifier.issn2587-2680
dc.identifier.issn2587-246X
dc.identifier.issue4
dc.identifier.startpage645
dc.identifier.trdizinid1215646
dc.identifier.urihttps://doi.org/10.17776/csj.1340869
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1215646
dc.identifier.urihttps://hdl.handle.net/20.500.12418/23488
dc.identifier.volume44
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofCumhuriyet Science Journal
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectOxidative damage
dc.subjectHydrogen Peroxide (H2O2)
dc.subjectIndatraline
dc.subjectC6 Glioma.
dc.titleInvestigation of the Effect of Indatraline on Oxidative Damage Induced by Hydrogen Peroxide in C6 Glioma Cell Line
dc.typeArticle

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu