Comparison of methods investigating Giardia intestinalis, Entamoeba histolytica, and Cryptosporidium spp. in stool samples of patients with diarrhea

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dc.contributor.authorKafa, Ayse Huemeyra Taskin
dc.contributor.authorCubuk, Fatih
dc.contributor.authorAslan, Rukiye
dc.contributor.authorHasbek, Mursit
dc.date.accessioned2024-10-26T18:05:33Z
dc.date.available2024-10-26T18:05:33Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBackground: Intestinal protozoan infections (IPIs), common all over the world, are an important public health problem, especially in developing countries. Different diagnostic methods are used for the diagnosis of causative agents in diarrhea cases. Objective: This study aims to analyze results of direct microscopy, coproantigen detection test, and PCR technique in diagnosis of G. intestinalis, E. histolytica/dispar, and Cryptosporidium spp. in stool samples of patients with diarrhea. Subjects and Methods: Fresh stool samples were collected from 683 patients complaining of diarrhea, and simultaneously examined by direct microscopy, commercial rapid diagnostic tests (RDTs) for detection of coproantigens, and molecularly using PCR technique. Results: The overall detection rate of parasites was 3.7% by direct microscopy, 6.6% by RDTs and 2% by PCR technique. Moderate, and weak fits were recorded between direct microscopy and RDTs results (Kappa=0.46, P<0.001), and between direct microscopy and PCR technique results (Kappa=0.236, P<0.001), respectively. No fit (Kappa=0.108, P=0.001) was recorded between coproantigen detection test and PCR technique results. Conclusion: It was concluded that direct microscopy and RDTs will be the correct approach in the first instance in the suspicion of IPIs. Despite high cost of PCR technique, it should be considered in differentiation between pathogenic and non-pathogenic amoeba, and genotyping of Cryptosporidium spp.
dc.identifier.doi10.21608/puj.2023.231013.1220
dc.identifier.endpage176
dc.identifier.issn1687-7942
dc.identifier.issn2090-2646
dc.identifier.issue3
dc.identifier.startpage171
dc.identifier.urihttps://doi.org/10.21608/puj.2023.231013.1220
dc.identifier.urihttps://hdl.handle.net/20.500.12418/29066
dc.identifier.volume16
dc.identifier.wosWOS:001145405400012
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherEgyptian Parasitologists United Soc - Epu
dc.relation.ispartofParasitologists United Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcoproantigens
dc.subjectdiagnostic methods
dc.subjectintestinal protozoa
dc.subjectmicroscopy
dc.subjectPCR
dc.subjectrapid diagnostic tests
dc.titleComparison of methods investigating Giardia intestinalis, Entamoeba histolytica, and Cryptosporidium spp. in stool samples of patients with diarrhea
dc.typeArticle

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