The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations

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dc.contributor.authorİnan, Zeynep Deniz Şahin
dc.contributor.authorPolat, Zübeyda Akın
dc.contributor.authorHamutoğlu, Rasim
dc.date.accessioned2024-10-26T17:34:00Z
dc.date.available2024-10-26T17:34:00Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThis study aimed to associate the possible cytotoxic and apoptotic effects of Botox (Allergan) and Dysport (Ipsen) and immunolocalization of matrix metalloproteinase (MMP) proteins with HCN2 cortical neuron cell line. Accordingly, cytotoxic potentials of Botox and Dysport were determined on different concentrations. Then, the apoptosis rates of these cells were evaluated by TUNEL method. MMP2, MMP7, and MMP9 proteins were also visualized using immunofluorescence method. There was a significant difference in cytotoxicity between those treated with 3.2, 6.4, and 12.8IU compared with the control. The Dysport 12.8 IU group was statistically more toxic than Botox group at the same concentration. Therefore, the number of apoptotic cells increased from 0.1 IU in Botox and 0.01 IU in Dysport groups compared to the control. The number of apoptotic cells was significantly higher in Dysport group at 1.6, 3.2, 6.4, and 12.8 IU concentrations than in Botox group. It was determined that MMPs increased gradually at the concentrations where the number of apoptotic cells was highest compared to the control group. As a result, we consider that it may be necessary to deal with the dose adjustment in Botox and Dysport applications, together with detailed studies to be carried out in the future.
dc.identifier.doi10.17776/csj.1180547
dc.identifier.endpage27
dc.identifier.issn2587-2680
dc.identifier.issn2587-246X
dc.identifier.issue1
dc.identifier.startpage19
dc.identifier.trdizinid1162877
dc.identifier.urihttps://doi.org/10.17776/csj.1180547
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1162877
dc.identifier.urihttps://hdl.handle.net/20.500.12418/23460
dc.identifier.volume44
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofCumhuriyet Science Journal
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectApoptosis
dc.subjectBotulinum neurotoxin A
dc.subjectHCN2
dc.subjectMMP
dc.subjectTUNEL.
dc.titleThe Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations
dc.typeArticle

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