Development of a liquid chromatographic method to monitorization of medazepam and lorazepam in plasma and its validation

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dc.contributor.authorAlpertenge, Melike
dc.contributor.authorDural, Emrah
dc.date.accessioned2024-10-26T17:52:48Z
dc.date.available2024-10-26T17:52:48Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThis study, it was aimed to develop a simple, sensitive and reliable high-performance liquid chromatographic method for simultaneous analysis of medazepam and lorazepam based on the solid-phase extraction from human blood. For the pretreatment of (500 µL) plasma sample, an efficient extraction method was developed and optimized. Separation was carried out with an ODS reverse phase C18 analytical column (150x4.0mm, 3µm). The composition of 20 mM KH2PO4 buffer and methyl cyanide (6:4, v/v) was employed as the mobile phase in the chromatographic system. The ultraviolet detector was set at 220nm. Determination of coefficients values was found as 0.9928 (r2) between 500-2500 ng/mL concentrations for medazepam and 0.9983 between 20-300 ng/mL for lorazepam. It was observed that the method has successful validation test results from accuracy, sensitivity, recovery, precision, and robustness in accordance with ICH Q2R1 guidelines. The method is recommended for monitoring blood levels of lorazepam and medazepam in toxicology laboratories. © 2023, Istanbul Medipol University. All rights reserved.
dc.identifier.doi10.23893/1307-2080.APS6116
dc.identifier.endpage255
dc.identifier.issn1307-2080
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85165952165
dc.identifier.scopusqualityQ3
dc.identifier.startpage235
dc.identifier.urihttps://doi.org/10.23893/1307-2080.APS6116
dc.identifier.urihttps://hdl.handle.net/20.500.12418/26527
dc.identifier.volume61
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherIstanbul Medipol University
dc.relation.ispartofActa Pharmaceutica Sciencia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHPLC-UV; lorazepam; Medazepam; method validation; solid-phase extraction
dc.titleDevelopment of a liquid chromatographic method to monitorization of medazepam and lorazepam in plasma and its validation
dc.typeArticle

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