Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells

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dc.contributor.authorYulak, Fatih
dc.date.accessioned2024-10-26T17:34:04Z
dc.date.available2024-10-26T17:34:04Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractIn this study, colchicine's cytotoxic effects on SNU-1 cells were examined, and a probable mechanism behind its cytotoxicity was revealed. According to the results of the study, colchicine displayed considerable cytotoxicity with an IC50 value of 14.81ng/ml when it was administered to the cells for 24 hours at different doses ranging from 5 to 100ng/ml. Furthermore, according to mechanistic studies, usege of colchicine significantly increased both early and late apoptotic cells in flow cytometry experiments. The late apoptotic cell population percentage in the control group (5.14 ± 1.27%) dramatically increased to 22.83 ± 1.38% in 14.81ng/ml colchicine treated cells. The early apoptotic cell population percentage in the control group (2.00 ± 1.12%) increased to 6.57 ± 2.35% in 14.81ng/ml colchicine treated cells. ELISA method was used to evaluate how colchicine affects the expression of pro- and anti-apoptotic proteins in SNU-1 cells. Colchicine treatment increased pro-apoptotic Bax and cleaved caspase 3 activities, while anti-apoptotic BCL-2 levels decreased. It is concluded that colchicine increases apoptosis in SNU-1 cells, which leads to an overall increase in cell death. Colchicine's promise as an anticancer drug to treat stomach cancer, however, needs additional research to be determined.
dc.identifier.doi10.17776/csj.1256954
dc.identifier.endpage267
dc.identifier.issn2587-2680
dc.identifier.issn2587-246X
dc.identifier.issue2
dc.identifier.startpage263
dc.identifier.trdizinid1186298
dc.identifier.urihttps://doi.org/10.17776/csj.1256954
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1186298
dc.identifier.urihttps://hdl.handle.net/20.500.12418/23478
dc.identifier.volume44
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofCumhuriyet Science Journal
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectColchicine
dc.subjectGastric cancer
dc.subjectAntiproliferative effect
dc.subjectApoptozis.
dc.titleInvestigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells
dc.typeArticle

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