Anticancer activity of sinapic acid by inducing apoptosis in HT-29 human colon cancer cell line

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dc.authoridyulak, fatih/0000-0003-3708-6752
dc.authoridHACISULEYMAN, LEVENT/0000-0002-4374-9025
dc.contributor.authorTastemur, Seyma
dc.contributor.authorHacisuleyman, Levent
dc.contributor.authorKarata, Ozhan
dc.contributor.authorYulak, Fatih
dc.contributor.authorAtaseven, Hilmi
dc.date.accessioned2024-10-26T18:07:52Z
dc.date.available2024-10-26T18:07:52Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractColorectal cancer is the third most lethal and fourth most commonly diagnosed cancer worldwide. Sinapic acid, a derivative of hydroxycinnamic acid, is a promising phytochemical exhibiting numerous pharmacological activities in various systems. It is a substantial chain-breaking antioxidant that operates as a radical scavenger. The aim of this research was to investigate the antiproliferative effect of sinapic acid on the HT-29 cell line besides the mechanisms underlying this activity. The effect of sinapic acid on the viability of HT-29 cell line was investigated using XTT assay. The levels of BCL-2, cleaved caspase 3, BAX, cleaved PARP, and 8-oxo-dG were measured using ELISA. Gamma-H2AX and cytochrome c expressions were assessed semiquantitatively using immunofluorescence staining. Sinapic acid at 200 & mu;m and higher doses produced a significant antiproliferative effect on HT-29 cells. The IC50 value was found to be 317.5 & mu;m for 24 h. Sinapic acid (317.5 & mu;m) significantly elevated cleaved caspase 3, BAX, cleaved PARP, and 8-oxo-dG levels. The levels of gamma-H2AX foci are significantly higher, while the levels of cytochrome c are lower in sinapic acid-treated HT-29 cells. These results indicate that sinapic acid has antiproliferative, apoptotic, and genotoxic effects on colon cancer cells.
dc.identifier.doi10.1139/cjpp-2022-0523
dc.identifier.endpage368
dc.identifier.issn0008-4212
dc.identifier.issn1205-7541
dc.identifier.issue7
dc.identifier.pmid36996488
dc.identifier.startpage361
dc.identifier.urihttps://doi.org/10.1139/cjpp-2022-0523
dc.identifier.urihttps://hdl.handle.net/20.500.12418/29719
dc.identifier.volume101
dc.identifier.wosWOS:001024358700004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherCanadian Science Publishing
dc.relation.ispartofCanadian Journal of Physiology and Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectsinapic acid
dc.subjectcolorectal cancer
dc.subjectHT-29 cell line
dc.subjectantiproliferative effect
dc.titleAnticancer activity of sinapic acid by inducing apoptosis in HT-29 human colon cancer cell line
dc.typeArticle

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