A Fluorescent Aptasensor for Sensitive and Selective Determination of Epigenetic Cancer Biomarker N1-Methyladenosine in Urine Samples

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dc.contributor.authorBagda, Esra
dc.contributor.authorBagda, Efkan
dc.contributor.authorLiu, Juewen
dc.date.accessioned2025-05-04T16:47:37Z
dc.date.available2025-05-04T16:47:37Z
dc.date.issued2025
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractN1-methyladenosine (m1A) level in urine increases in the presence of cancer and is associated with the tumor size and stage. In the present study, we aimed to develop a method for rapid, sensitive and accurate determination of m1A in urine samples. The capture systematic evolution of ligands by exponential enrichment (SELEX) method was used to isolate aptamers that could selectively bind to m1A. We successfully isolated two sequences that have high selectivity toward m1A. The affinities against m1A were determined by isothermal titration calorimetry (ITC) and thioflavin T (ThT) assays. The N1MA1a aptamer has a Kd of 1.9 +/- 0.1 mu m determined by the ThT assay and 0.75 +/- 0.04 mu m determined by ITC. A strand-displacement biosensor was designed by labeling the aptamer with a carboxy fluorescein (FAM) and hybridizing it with a quencher-labeled complementary DNA strand. Using this biosensing system, m1A was detected with a detection limit of 1.9 mu m. The system shows high selectivity to m1A and high tolerance to adenosine, cytidine, guanosine, thymidine, uridine and N6-methyladenosine (m6A) as well as urine constituents at their real levels in urine. The sensor has been applied to five different human urine samples showing quantitative recovery values, which indicates practical potential of this aptamer-based biosensor.
dc.description.sponsorshipScientific and Technological Research Council of Turkiye-TUBIdot;TAK, 2219 - International Postdoctoral Research Fellowship Program; Natural Sciences and Engineering Research Council of Canada (NSERC)
dc.description.sponsorshipEsra Ba & gbreve;da is supported by the Scientific and Technological Research Council of Turkiye-TUB & Idot;TAK, 2219 - International Postdoctoral Research Fellowship Program for Turkish Citizens to visit the University of Waterloo. The authors acknowledge funding for this work from The Natural Sciences and Engineering Research Council of Canada (NSERC).
dc.identifier.doi10.1002/chem.202500105
dc.identifier.issn0947-6539
dc.identifier.issn1521-3765
dc.identifier.pmid40178378
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/chem.202500105
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35668
dc.identifier.wosWOS:001467521500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofChemistry-A European Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250504
dc.subjectAptamer
dc.subjectBiosensor
dc.subjectCapture SELEX
dc.subjectN1-methyladenosine
dc.subjectUrine
dc.titleA Fluorescent Aptasensor for Sensitive and Selective Determination of Epigenetic Cancer Biomarker N1-Methyladenosine in Urine Samples
dc.typeArticle

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