Observations on Acanthamoeba trophozoites in axenic cultures and their staining characteristics with different stains

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dc.contributor.authorPolat, Zübeyde Akin
dc.contributor.authorOzçelik, Semra
dc.contributor.authorVural, Ayşe
dc.contributor.authorSaygi, Gülendame
dc.date.accessioned2024-10-26T17:54:10Z
dc.date.available2024-10-26T17:54:10Z
dc.date.issued2007
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractAcanthamoeba spp. are among the most prevalent protozoa found in the environment. The species of this genus are the causative agents of granulomatous amebic encephalitis (GAE), a fatal disease of the central nervous system (CNS), and amebic keratitis (AK), a painful sight-threatening disease of the eye. In this study we have used two species of Acanthamoeba, Acanthamoeba castellanii and A. hatchetti, both were obtained from Vienna, Austria. They were cultivated on non-nutritious agar seeded with Escherichia coli and PPYG (protease peptone-yeast extract-glucose) medium. Our aim was to concentrate on three points in relation to the trophozoites and cysts stages of these species as follows: (i) to observe their morphology, (ii). to confirm our previous observation of a canal between two trophozoites. The bridge-like connection between these trophozoites greatly resembled the one that can be observed in conjugation during an exchange of genetic material. Two tro-phozoites with a bridge-like extension between them keep their position for at least 200 minutes. (iii). to detect the reactions of trophozoites to various stains. According to our findings in regard to these three points: (i). trophozoites with more than one nucleus are often seen in axenic cultures. (ii). This resembles a type of conjugation with a transfer of genetic material between two trophozoites. Certainly, this needs further investigation using more sophisticated methods. (iii). trophozoites equally stained well with Heidenhain's iron haematoxylin, Giemsa, PAS, Masson Trichrome, and Toludin-O stains. However, our results with reticulin, PAP, Van Gison, Musicarmine and Orsein stains were not satisfactory.
dc.identifier.endpage13
dc.identifier.issn1300-6320
dc.identifier.issue1
dc.identifier.pmid17471404
dc.identifier.scopus2-s2.0-38449106370
dc.identifier.scopusqualityQ4
dc.identifier.startpage7
dc.identifier.urihttps://hdl.handle.net/20.500.12418/27030
dc.identifier.volume31
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isotr
dc.relation.ispartofTürkiye parazitolojii dergisi / Türkiye Parazitoloji Derne?i = Acta parasitologica Turcica / Turkish Society for Parasitology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcanthamoeba; Amebiasis; Animals; Coloring Agents; Conjugation, Genetic; Staining and Labeling; Trophozoites; coloring agent; Acanthamoeba; amebiasis; animal; article; bacterium conjugation; classification; cytology; drug effect; methodology; parasitology; physiology; staining; trophozoite
dc.titleObservations on Acanthamoeba trophozoites in axenic cultures and their staining characteristics with different stains
dc.title.alternativeAksenik kültürlerde Acanthamoeba trofozoitleri üzerindeki gözlemler ve bunlarin farkli boyalarla boyanma özellikleri.
dc.typeArticle

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