Study of lipase enzym activity with Candida utilis yeast in different media condition

Basit Öğe Kaydı
dc.contributor.authorKeklikcio?lu Çakmak, Neçe
dc.contributor.authorAçikel, Ünsal
dc.date.accessioned2024-10-26T17:54:08Z
dc.date.available2024-10-26T17:54:08Z
dc.date.issued2015
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractLipases (triacylglycerol hydrolases, EC 3.1.1.3) are hydrolytic enzymes that can catalyze the hydrolysis of the ester bond of long chain acylglycerols at the oil-water interface. In this study; the effects of initial pH, initial sucrose concentration, activators and inhibitors on Candida utilis yeast, lipase enzym are searched. It is qualified that the optimum pH value for growth rate of Candida utilis yeast and production of lipase enzym is 4. Molasses which is a remnant of sugar industry is used as a main carbon source in nutrient media that is prepared for experimental studies in which lipase enzym activity is worked out. In the media in which molasses is used as a main carbon source, it is observed that lipase enzym activity increases with the increase of initial sucrose concentration. Soybean oil, corn oil, olive oil, sunflower oil and canola oil are added to the nutrient media and when the additive effect of enzym activity is observed it is found out that the highest activity increase is 788, 5 U/L with the addition of 1, 25% soybean oil. The result is that; Cu(II) and Ni(II) metal ions in the fermantation media lower the lipase activity severly and besides, lowering effect of Ni(II) is much more than that of Cu(II).
dc.identifier.endpage485
dc.identifier.issn1300-1884
dc.identifier.issue3
dc.identifier.scopus2-s2.0-84942944830
dc.identifier.scopusqualityQ2
dc.identifier.startpage475
dc.identifier.urihttps://hdl.handle.net/20.500.12418/26994
dc.identifier.volume30
dc.indekslendigikaynakScopus
dc.language.isotr
dc.publisherGazi Universitesi Muhendislik-Mimarlik
dc.relation.ispartofJournal of the Faculty of Engineering and Architecture of Gazi University
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCandida utilis; Cu (II); Inhibition; Lipase; Molasses; Ni (II)
dc.titleStudy of lipase enzym activity with Candida utilis yeast in different media condition
dc.title.alternativeCandida utilis Mayasiyla lipaz enzimi aktivitesinin farkli ortam koşullarinda incelenmesi
dc.typeArticle

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu