Scavenging Effect, Chemical Composition and Antispasmodic Activity of the Essential Oil of Origanum Onites L.

Scavenging were determined in vitro. GC-MS analysis that was been carried out upon the essentialoil revealed 31 different composites which equal to 94.60% in which Carvacrol (49.01%), linalool(13.3%) were found as major components. Free radical scavenging capacities of the essential oilwere measured by 2,2-diphenyl-1-pikrilhidrazil (DPPH) assay and via ?-carotene linoleic acidexperiment were determined. It showed IC50 value of Origanum onites essential oil was beenidentified as 430 mg/mL, the IC50 value for BHT was 19.8 mg/mL, the ?-carotene linoleic acid assayalso showed the 80% inhibition with the essential oil of Origanum onites.it showed very strongantioxidant activity. In addition, dose dependently (0.1, 0.5, 1.0 and 2 mg/mL) inhibits thecontraction induced by, it was able to inhibit the rat ileum muscle by 2 mg/mL to 100%.