Development of quantum dot-phthalocyanine integrated G-quadruplex/double-stranded DNA biosensor

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dc.contributor.authorTopcu, Ayşe
dc.contributor.authorBağda, Esra
dc.contributor.authorOymak, Tülay
dc.contributor.authorDurmuş, Mahmut
dc.date.accessioned2023-06-22T13:02:41Z
dc.date.available2023-06-22T13:02:41Z
dc.date.issued1 October 2022tr
dc.departmentEczacılık Fakültesitr
dc.description.abstractn the present study, the phthalocyanine (Pc) integrated mercaptopropionic acid capped quantum dot (mpa@QD) biosensor has been developed for the quantitative determination of G-quadruplex and double-stranded DNA. The working principle of the developed biosensor platform is based on the quenching of the emission signal of the mpa@QD in the presence of Pc (closed position) and the recovery of the fluorescence signal in the presence of DNA (open position). The parameters affecting biosensor performance, such as Pc type and concentration, were optimized. Since the developed biosensor aimed to determine G-quadruplex and double-stranded DNA in biological samples, the effect of common ions (such as Na+, Mg2+) and serum albumin found in many biological matrices on the biosensor performance were examined. The effect of common ions on biosensor signal was negligible, except Zn2+. The analytical properties of the biosensor, such as linear range, calibration sensitivity, relative standard deviation %, the limit of detection, and quantification, were determined. The limit of detection and quantification values were found 0.055 μM and 0.18 μM for AS1411, 0.061 μM and 0.20 μM for Tel21, 0.038 μM and 0.13 μM for Tel45 and 0.091 μM and 0.30 μM for ctDNA. Several different synthetic samples were prepared. The spiked synthetic samples such as mammalian cell medium were used to evaluate the analytical performance of Pc-mpa@QD. All synthetic samples were prepared with polyethylene glycol, which resembles biological samples' crowded environment.tr
dc.identifier.citationAys¸e Topcu a , Esra Bagda ˘ a,* , Tülay Oymak a , Mahmut Durmus¸ b a Department of Basic Pharmaceutical Sciences, Analytical Chemistry Division, Faculty of Pharmacy, Sivas Cumhuriyet University, 58140, Sivas, Turkey b Gebze Technical University, Department of Chemistry, Gebze, 41400, Kocaeli, Turkeytr
dc.identifier.pmid35750250en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12418/13982
dc.identifier.wosWOS:000860475500001en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıtr
dc.rightsinfo:eu-repo/semantics/embargoedAccesstr
dc.subjectG-quadruplexQuantum dotPhthalocyanineBiosensortr
dc.titleDevelopment of quantum dot-phthalocyanine integrated G-quadruplex/double-stranded DNA biosensoren_US
dc.typeArticleen_US

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