Determination of phenytoin in human plasma by a validated hplc method: Application to therapeutic drug monitoring study running title: Measurement of phenytoin in plasma by a novel hplc method

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dc.contributor.authorDural, Emrah
dc.contributor.authorBolayır, Aslı
dc.contributor.authorÇiğdem, Burhanettin
dc.date.accessioned2024-10-26T17:52:48Z
dc.date.available2024-10-26T17:52:48Z
dc.date.issued2021
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThe aim of this study was to develop a simple and reliable HPLC method for the determination of phenytoin (PHT) in human plasma. Accuracy (RE%) were determined between (-0.93%) to 2.49% and precision (RSD%) values was ?7.94. The quantitation limit was 3.54 µg/mL and recovery was found between 82.15% and 101.06%. The method was applied to real plasma samples (n = 7). Plasma-PHT levels were found between 1.12 and 18.76 µg/mL (9.52 ± 7.78, mean±SD). Both the plasma and dose-rated plasma results of PHT showed so high RSD% which were between 81.74% and 89.61%. In addition plasma-PHT levels were outside the recom-mended treatment range in 4 of the 7 patients (57.14%) examined, and also surpris-ingly PHT could not be detected in a patient’s plasma. This procedure is relatively simple, precise, and applicable for routine therapeutic drug monitorization of PHT in neurology clinics or toxicological analyses in reference laboratories. © 2021, İstanbul Medipol University. All rights reserved.
dc.description.sponsorshipSivas Cumhuriyet Üniversitesi
dc.identifier.doi10.23893/1307-2080.APS.05909
dc.identifier.endpage704
dc.identifier.issn1307-2080
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85100543358
dc.identifier.scopusqualityQ3
dc.identifier.startpage683
dc.identifier.urihttps://doi.org/10.23893/1307-2080.APS.05909
dc.identifier.urihttps://hdl.handle.net/20.500.12418/26526
dc.identifier.volume59
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherİstanbul Medipol University
dc.relation.ispartofActa Pharmaceutica Sciencia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHPLC-UV; Human plasma; Method validation; Phenytoin; Therapeutic drug monitoring
dc.titleDetermination of phenytoin in human plasma by a validated hplc method: Application to therapeutic drug monitoring study running title: Measurement of phenytoin in plasma by a novel hplc method
dc.typeArticle

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