Investigation of Interaction of Bismarck Brown Y- Palladium Complex with AS1411 G-quadruplex Aptamer

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dc.contributor.authorBağda, Esra
dc.contributor.authorDurak, Rümeysa
dc.contributor.authorBağda, Efkan
dc.contributor.authorDuman, Didem
dc.contributor.authorAyhan, Ebubekir
dc.date.accessioned2024-10-26T17:42:50Z
dc.date.available2024-10-26T17:42:50Z
dc.date.issued2022
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractIncreased metabolic activity and metastasis are the main and most known characteristics of cancer cells. Increased activity of the cells results in an increase in the transcription, translation, and replication rate of DNA, hence the probability of the formation of G-quadruplex structures increases. The stabilization or destabilization of G-quadruplex with various ligands may cause disruptions in cell proliferation. So, stabilization or destabilization of these secondary structures is important cancer therapy approach. In the present study, Bismarck brown Y-Pd complex was formed in an easy, one-step mixing method. The spectral characteristics and stoichiometry of the BBY-Pd2 were investigated UV-Vis spectrophotometrically. The interaction of the BBY-Pd2 complex with the AS1411 G-quadruplex structure was investigated with spectrophotometric titration. The binding constants were found as 4.38 (± 1.96) × 104 M-1. The effect of the complex on the G-quadruplex conformation of AS1411 was investigated by using circular dichroism (CD) spectrophotometer. The existence of interactions was further supported by DNA polymerase stop assay using a high-sensitivity LED-induced fluorescence detector Qsep100 capillary gel electrophoresis system.
dc.identifier.doi10.51435/turkjac.1203081
dc.identifier.endpage122
dc.identifier.issn2687-6698
dc.identifier.issue2
dc.identifier.startpage116
dc.identifier.trdizinid1154742
dc.identifier.urihttps://doi.org/10.51435/turkjac.1203081
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1154742
dc.identifier.urihttps://hdl.handle.net/20.500.12418/24863
dc.identifier.volume4
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTurkish Journal of Analytical Chemistry (Online)
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectG-quadruplex DNA
dc.subjectBismarck brown Y
dc.subjectPalladium
dc.subjectCD spectroscopy
dc.subjectcapillary gel electrophoresis
dc.subjectAS1411
dc.titleInvestigation of Interaction of Bismarck Brown Y- Palladium Complex with AS1411 G-quadruplex Aptamer
dc.typeArticle

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