Molecular survey and phylogenetic analysis of bee pathogens; with a note first detection of Apis mellifera Filamentous Virus, Varroa destructor virus-1, Apis rhabdovirus-1, and Apis rhabdovirus-2 in Türkiye

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dc.authoridALTAY, KURSAT/0000-0002-5288-1239
dc.authoridKALIN, Recep/0000-0002-9173-9550
dc.contributor.authorAltay, Kursat
dc.contributor.authorIsidan, Hakan
dc.contributor.authorErol, Ufuk
dc.contributor.authorTuran, Turhan
dc.contributor.authorSahin, Omer Faruk
dc.contributor.authorKalin, Recep
dc.contributor.authorAtasoy, Mustafa Ozan
dc.date.accessioned2025-05-04T16:46:56Z
dc.date.available2025-05-04T16:46:56Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractTurkey is one of the most important bee centers in the world, but there is limited information about honey bee pathogens in the country. This study aimed to investigate bee pathogens using microscopic and molecular techniques (PCR, RT-PCR, nested-PCR, and sequencing) in Sivas province, the second-biggest beekeeping center in Turkey. For this purpose, bee samples were obtained from 149 bee colonies belonging to 74 local beekeepers in eight districts of the Sivas province. The bee samples were examined for pathogens, and at least one pathogen was detected in 148 bee colonies. In this study, a single infection was determined in 26.17% (39/149) of the bee samples, while the prevalence of co-infection was 73.15% (109/149). Nosema ceranae, Varroa destructor haplotype-K, Apis mellifera Filamentous Virus, Deformed wing virus, Varroa destructor virus-1, Lake Sinai virus-2, Apis rhabdovirus-1, and Apis rhabdovirus-2 were detected. Apis mellifera Filamentous Virus, Varroa destructor virus-1, Apis rhabdovirus-1, and Apis rhabdovirus-2 were found for the first time in Turkey with the current study. Furthermore, nucleotide sequence analysis and phylogeny relationships of bee pathogens obtained from samples were done, and sequences were deposited to GenBank. This study provides molecular data on the presence and prevalence of important bee pathogens in the region. As the prevalence of many pathogens has been determined, especially the existence of viral new species has been revealed, the effects of the species on beekeeping should be studied, and beekeepers should take effective control measures to reduce the effects of these pathogens.
dc.description.sponsorshipScientific Research Project Fund of Sivas Cumhuriyet University
dc.description.sponsorshipThe authors would like to thank Sivas Beekeepers Association and beekeepers for their kind help during sample collection.
dc.identifier.doi10.1080/00218839.2024.2423456
dc.identifier.issn0021-8839
dc.identifier.issn2078-6913
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1080/00218839.2024.2423456
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35419
dc.identifier.wosWOS:001353292500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofJournal of Apicultural Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250504
dc.subjectBee viruses
dc.subjectV. destructor haplotype-K
dc.subjectNosema ceranae
dc.subjectmolecular identification
dc.subjectphylogeny
dc.titleMolecular survey and phylogenetic analysis of bee pathogens; with a note first detection of Apis mellifera Filamentous Virus, Varroa destructor virus-1, Apis rhabdovirus-1, and Apis rhabdovirus-2 in Türkiye
dc.typeArticle

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