Overcoming Drug Resistance: P-Gp Inhibitor Verapamil Enhances Temozolomide's Antiproliferative Effect in Neuroblastoma Cells

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dc.contributor.authorKarademir, Mustafa
dc.contributor.authorAltun, Ahmet
dc.date.accessioned2024-10-26T18:04:15Z
dc.date.available2024-10-26T18:04:15Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractNeuroblastoma is one of the most common and deadly childhood solid tumors. P-glycoprotein (P-gp) pump plays a role in developing resistance to many chemotherapeutic agents. The high expression of P-gp is associated with poor prognosis in drug resistance and neuroblastoma treatment. We aimed to evaluate the anticancer effect of Temozolomide (TMZ) on the SH-SY5Y human neuroblastoma cell line in the presence of P-gp inhibitor Verapamil (VER). In the present study, the antiproliferative effect of TMZ on SH-SY5Y cells alone and in combination with VER was evaluated using a colorimetric XTT viability test.SH-SY5Y cells were seeded to 96 well plates at 10 000 cells/well. TMZ (100 mu M-10 mM) and VER (0.25-10 mu g/mL) were applied to the cells alone first, then XTT measurements were performed after 24 and 48 h. In the study's second phase, VER was applied to the cells at the fixed concentration of 2.5 mu g/mL to block P-gp pumps, then increasing concentrations of TMZ were applied to the cells in the presence of VER. The current study showed that in addition to cytotoxic effects, VER + TMZ administration accelerated apoptosis in SH-SY5Y cells compared to TMZ utilization alone. The wound healing assay demonstrated that VER + TMZ combination also inhibited cell migration. Considering this evidence, combining TMZ with VER in the neuroblastoma cell line may inhibit migration and cell proliferation via the apoptosis pathway and produce a substantial anticancer effect.
dc.description.sponsorshipSivas Cumhuriyet University Scientific Research Projects Department [T-832]
dc.description.sponsorshipThis study was funded by Sivas Cumhuriyet University Scientific Research Projects Department (Project number T-832).
dc.identifier.doi10.1134/S1819712424010082
dc.identifier.endpage124
dc.identifier.issn1819-7124
dc.identifier.issn1819-7132
dc.identifier.issue1
dc.identifier.startpage119
dc.identifier.urihttps://doi.org/10.1134/S1819712424010082
dc.identifier.urihttps://hdl.handle.net/20.500.12418/28839
dc.identifier.volume18
dc.identifier.wosWOS:001232700000016
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherMaik Nauka/Interperiodica/Springer
dc.relation.ispartofNeurochemical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectSH-SY5Y
dc.subjectTemozolomide
dc.subjectVerapamil
dc.subjectP-glycoprotein Pump Inhibitor
dc.subjectCytotoxicity
dc.titleOvercoming Drug Resistance: P-Gp Inhibitor Verapamil Enhances Temozolomide's Antiproliferative Effect in Neuroblastoma Cells
dc.typeArticle

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