Effects of Detergents, Ions, and Organic Solvents on the Activity of Four Bacillus clausii Pectinases

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dc.contributor.authorBerber, Sevinç
dc.contributor.authorÇetinkaya, Serap
dc.date.accessioned2024-10-26T17:44:20Z
dc.date.available2024-10-26T17:44:20Z
dc.date.issued2020
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractFour different pectinolytic enzyme activity essays were employed to detect different pectinases ofBacillus clausii from an extracellular protein cocktail. The mixture was obtained by ethanol precipitation.Dinitrosalicylic acid (DNS) and sodium hydroxide (NaOH) titrimetric methods were used for thepectinase assays. Each of the essays produced a positive result, indicating the presence of pectate lyase(PGL), pectin lyase (PL), pectin methyl esterase (PME), and polygalacturonase (PG). Activities of theseenzymes were then assessed at differing concentrations of NaCl, detergents (SDS and tweens), ionchelators, and organic solvents. Results, presented as histograms, were the indication of original findings,as most of them were new to the relevant literature.
dc.identifier.doi10.780314/cbayarfbe.780314
dc.identifier.endpage392
dc.identifier.issn1305-130X
dc.identifier.issn1305-1385
dc.identifier.issue4
dc.identifier.startpage387
dc.identifier.trdizinid458109
dc.identifier.urihttps://doi.org/10.780314/cbayarfbe.780314
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/458109
dc.identifier.urihttps://hdl.handle.net/20.500.12418/25219
dc.identifier.volume16
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofCelal Bayar Üniversitesi Fen Bilimleri Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleEffects of Detergents, Ions, and Organic Solvents on the Activity of Four Bacillus clausii Pectinases
dc.typeArticle

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